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Literature summary for 2.5.1.9 extracted from

  • Ramsperger, A.; Augustin, M.; Schott, A.K.; Gerhardt, S.; Krojer, T.; Eisenreich, W.; Illarionov, B.; Cushman, M.; Bacher, A.; Huber, R.; Fischer, M.
    Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor: stereochemical implications (2006), J. Biol. Chem., 281, 1224-1232.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of wild-type enzyme are grown at 18°C using the sitting drop vapor diffusion method by mixing equal amounts of protein (6 mg/ml) in 100 mM potassium phosphate, pH 7.0, containing 30 mM Tris and 2 mM dithiothreitol with a reservoir solution containing 0.1 M HEPES, pH 7.0, and 40% 2-methyl-2,4-pentanediol. Crystal structures of the enzyme and its complex with the substrate analog inhibitor, 6,7-dioxo-8-ribityllumazine Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58584
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Methanocaldococcus jannaschii DSM 2661 Q58584
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Subunits

Subunits Comment Organism
pentamer
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Methanocaldococcus jannaschii