Cloned (Comment) | Organism |
---|---|
expression of beta60 capsid | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
molecular modeling of inhibitors to the active site | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butanoic acid | comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli | |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butyl dihydrogen phosphate | comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli | |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]propyl dihydrogen phosphate | competitive, comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli | |
5-(hexyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli | |
5-(pentyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli | |
6-methyl-7-methylidene-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,8-dihydropyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione | competitive, comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli | |
9-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,9-dihydro-1H-purine-2,6,8(3H)-trione | competitive, comparison with inhibition of Bacillus subtilis lumazine synthase | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine | - |
Escherichia coli | riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00061 | - |
9-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,9-dihydro-1H-purine-2,6,8(3H)-trione | pH 7.5, 37°C | Escherichia coli | |
0.02 | - |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butanoic acid | pH 7.5, 37°C | Escherichia coli | |
0.15 | - |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]butyl dihydrogen phosphate | pH 7.5, 37°C | Escherichia coli | |
0.65 | - |
6-methyl-7-methylidene-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-7,8-dihydropyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione | pH 7.5, 37°C | Escherichia coli | |
1 | - |
5-(hexyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | pH 7.5, 37°C | Escherichia coli | |
1 | - |
5-(pentyl 6-dihydrogen phosphate)-6-([(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]amino)pyrimidine-2,4(1H,3H)-dione | pH 7.5, 37°C | Escherichia coli | |
1.6 | - |
4-[2,4,7-trioxo-8-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-1,2,3,4,7,8-hexahydropteridin-6-yl]propyl dihydrogen phosphate | pH 7.5, 37°C | Escherichia coli |