Literature summary for 2.5.1.9 extracted from

Gerhardt, S.; Schott, A.K.; Kairies, N.; Cushman, M.; Illarionov, B.; Eisenreich, W.; Bacher, A.; Huber, R.; Steinbacher, S.; Fischer, M.
Studies on the reaction mechanism of riboflavin synthase: X-ray crystal structure of a complex with 6-carboxyethyl-7-oxo-8-ribityllumazine (2002), Structure, 10, 1371-1381.

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization of enzyme complexed with 6-carboxyethyl-7-oxo-8-ribityllumazine, sitting drop vapour diffusion method against equal amounts of reservoir solution containing 0.1 M bicine, pH 9.0, 65% v/v 2-mehyl-2,4-pentanediol, enzyme solution: 9 mg/ml, 20 mM TrisHCl, pH 7.0, 0.1 M KCl, 10 molar excess of solid 8, X-ray structure determination and analysis, structure model building and refinement, overview	Schizosaccharomyces pombe

Crystallization (Comment)

Organism

crystallization of enzyme complexed with 6-carboxyethyl-7-oxo-8-ribityllumazine, sitting drop vapour diffusion method against equal amounts of reservoir solution containing 0.1 M bicine, pH 9.0, 65% v/v 2-mehyl-2,4-pentanediol, enzyme solution: 9 mg/ml, 20 mM TrisHCl, pH 7.0, 0.1 M KCl, 10 molar excess of solid 8, X-ray structure determination and analysis, structure model building and refinement, overview

Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine	Schizosaccharomyces pombe	-	riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	Q9Y7P0	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	reaction mechanism	Schizosaccharomyces pombe	a
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	substrate binding site	Schizosaccharomyces pombe	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine	-	Schizosaccharomyces pombe	riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine	-	?
additional information	structure-function relationship	Schizosaccharomyces pombe	?	-	?

Subunits

Subunits	Comment	Organism
More	structure model, biological implication	Schizosaccharomyces pombe