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Literature summary for 2.5.1.9 extracted from

  • Suzuki, Y.; Terai, Y.; Abe, S.
    Purification and some properties of riboflavin synthetase from Bacillus stearothermophilus ATCC 8005 (1978), Appl. Environ. Microbiol., 35, 258-263.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information no requirement for a cofactor Geobacillus stearothermophilus

General Stability

General Stability Organism
stable at 26°C in 4 M urea for 18 h, 4 M urea completely inactivates at 55°C Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Geobacillus stearothermophilus
6-Methyl-7-hydroxy-8-ribityllumazine
-
Geobacillus stearothermophilus
Ca2+
-
Geobacillus stearothermophilus
Cu2+
-
Geobacillus stearothermophilus
Hg2+
-
Geobacillus stearothermophilus
iodoacetamide
-
Geobacillus stearothermophilus
Mg2+
-
Geobacillus stearothermophilus
Mn2+
-
Geobacillus stearothermophilus
additional information no effect by Ba2+, Co2+, EDTA Geobacillus stearothermophilus
p-chloromercuribenzoate
-
Geobacillus stearothermophilus
Zn2+
-
Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km is temperature-dependent Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect by Ba2+, Co2+ Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
345000
-
gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine Geobacillus stearothermophilus
-
riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
strain ATCC 8005
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.011
-
purified enzyme Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
4°C, 6 months, no loss of activity Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-8-ribityllumazine + 6,7-dimethyl-8-ribityllumazine
-
Geobacillus stearothermophilus riboflavin + 4-(1'-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 95 50°C: about 10% of activity maximum, 80°C: about 30% of activity maximum, 95°C: activity maximum Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
24 h, no loss of activity, without substrate Geobacillus stearothermophilus
85
-
10 min, complete loss of activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7.2
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.4 8.8 about 50% of activity maximum at pH 5.4 and 8.8 Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 26°C, 18 h, stable Geobacillus stearothermophilus
6.3 7.6 55°C, 18 h, stable Geobacillus stearothermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.18
-
6-Methyl-7-hydroxy-8-ribityllumazine
-
Geobacillus stearothermophilus