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Literature summary for 2.5.1.88 extracted from

  • Ambo, T.; Noike, M.; Kurokawa, H.; Koyama, T.
    Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca (2009), J. Biosci. Bioeng., 107, 620-622.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate about 35% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + di-trans,poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
(2Z,6E)-farnesyl diphosphate + n isopentenyl diphosphate highest activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + trans,poly-cis-polyprenyl diphosphate n = 9-11. Broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
geranyl diphosphate + n isopentenyl diphosphate about 5% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + trans,poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
geranylgeranyl diphosphate + n isopentenyl diphosphate about 30% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + tri-trans,poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
neryl diphosphate + n isopentenyl diphosphate about 10% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Thermobifida fusca