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Literature summary for 2.5.1.87 extracted from

  • Juchimiuk, M.; Orlowski, J.; Gawarecka, K.; Swiezewska, E.; Ernst, J.F.; Palamarczyk, G.
    Candida albicans cis-prenyltransferase Rer2 is required for protein glycosylation, cell wall integrity and hypha formation (2014), Fungal Genet. Biol., 69, 1-12.
    View publication on PubMed
Search for more literature for 2.5.1.87

Cloned(Commentary)

Cloned (Comment) Organism
gene rer2, DNA and amino acid sequence determination and analysis, recombinant expression in Saccharomyces cerevisiae strain KG219, expression of CaRER2 under control of the MET3 promoter in mutant strain JOS14 Candida albicans

Protein Variants

Protein Variants Comment Organism
additional information construction of the PMET3RER2/rer2D mutant strain JOS18, and of ura3D::imm434/ura3D::imm434 RER2/rer2D::hisG mutant strain JOS14, and of ura3D::imm434/ura3D::imm434 RER2/rer2D::hisG-URA3-hisG mutant strain JOS13 Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
 Candida albicans 5618
-
membrane
-
 Candida albicans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
-
 Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate Candida albicans cis-prenyltransferase activity of the Candida albicans Rer2 homologue n diphosphate + ditrans,polycis-polyprenyl diphosphate n =10-55 ?
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate Candida albicans ATCC MYA-2876 cis-prenyltransferase activity of the Candida albicans Rer2 homologue n diphosphate + ditrans,polycis-polyprenyl diphosphate n =10-55 ?

Organism

Organism UniProt Comment Textmining
Candida albicans Q5AJZ3 gene rer2
-
Candida albicans ATCC MYA-2876 Q5AJZ3 gene rer2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate cis-prenyltransferase activity of the Candida albicans Rer2 homologue Candida albicans n diphosphate + ditrans,polycis-polyprenyl diphosphate n =10-55 ?
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate cis-prenyltransferase activity of the Candida albicans Rer2 homologue Candida albicans ATCC MYA-2876 n diphosphate + ditrans,polycis-polyprenyl diphosphate n =10-55 ?
additional information dolichol synthesis using isopentenyl diphosphate Candida albicans ?
-
 ?
additional information dolichol synthesis using isopentenyl diphosphate Candida albicans ATCC MYA-2876 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, about, sequence calculation Candida albicans

Synonyms

Synonyms Comment Organism
cis-prenyltransferase
-
 Candida albicans
Rer2p
-
 Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Candida albicans

General Information

General Information Comment Organism
malfunction the morphological differentiation of a Candida albicans cis-prenyltransferase mutant is impaired Candida albicans
metabolism cis-Prenyltransferase is the first enzyme of the mevalonate pathway committed to the biosynthesis of dolichol in eukaryotes Candida albicans
physiological function cis-prenyltransferase Rer2 is required for protein glycosylation, cell wall integrity and hypha formation. Dolichols are essential not only for protein glycosylation and cell wall integrity but also for growth and development of Candida albicans Candida albicans