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Literature summary for 2.5.1.87 extracted from

  • Kurisaki, A.; Sagami, H.; Ogura, K.
    Proteolytic release of dehydrodolichyl diphosphate synthase from pig testis microsomes (1996), Biosci. Biotechnol. Biochem., 60, 1109-1114.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
deoxycholate microsomal enzyme and enzyme released from microsomes are maximally activated at 5 mM. In the absence of deoxycholate, the released enzyme is inactive, while the microsomal enzyme has significant activity Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
KCl 0.4 M, more than 90% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000532
-
isopentenyl diphosphate pH 8.5, 37°C, enzyme released from microsomes Sus scrofa
0.000538
-
isopentenyl diphosphate pH 8.5, 37°C, microsomal enzyme Sus scrofa
0.013
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, microsomal enzyme Sus scrofa
0.0149
-
(2E,6E)-farnesyl diphosphate pH 8.5, 37°C, enzyme released from microsomes Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 1 mM Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
gel filtration of the released enzyme gives a peak of dehydrodolichyl diphosphate synthase activity, which appears between 150000 Da and 50000 Da molecular mass markers Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Sus scrofa
-

Storage Stability

Storage Stability Organism
-80°C, presence of glycerol is indispensable Sus scrofa
4°C, 5 days, 20% glycerol, more than 60% of enzyme activity is retained Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate
-
Sus scrofa diphosphate + di-trans,poly-cis-polyprenyl diphosphate
-
?

Synonyms

Synonyms Comment Organism
dehydrodolichyl diphosphate synthase
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Sus scrofa