Literature summary for 2.5.1.87 extracted from

Poznanski, J.; Szkopinska, A.
Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae (2007), Biopolymers, 86, 155-164.

Crystallization (Commentary)

Crystallization (Comment)	Organism
on the basis of Escherichia coli undecaprenyl diphosphate synthase crystallographic structure the yeast Rer2p model is constructed. In the model three amino acid residues inserted into the sequence corresponding to the floor region of the tunnel extends the bottom loop what results in the required increase of the tunnel volume. Thermal fluctuations of this loop occasionally create a hole in the tunnel floor, making escape of polyprenol omega end out of the tunnel possible what switches off the control mechanism of product length thereby allowing a practically unlimited elongation process leading to an exponential distribution of longer chain polyprenols	Saccharomyces cerevisiae

Crystallization (Comment)

Organism

on the basis of Escherichia coli undecaprenyl diphosphate synthase crystallographic structure the yeast Rer2p model is constructed. In the model three amino acid residues inserted into the sequence corresponding to the floor region of the tunnel extends the bottom loop what results in the required increase of the tunnel volume. Thermal fluctuations of this loop occasionally create a hole in the tunnel floor, making escape of polyprenol omega end out of the tunnel possible what switches off the control mechanism of product length thereby allowing a practically unlimited elongation process leading to an exponential distribution of longer chain polyprenols

Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate	Saccharomyces cerevisiae	Rer2p Z-prenyltransferase synthesizes a well-defined family of polyprenols of 1318 isoprene residues with dominating C80 (16 isoprene residues) extending to C120. Two polyisoprenoid families with dominating C80 and C110 indicating the activity of both yeast Z-prenyltransferases Rer2p and Srt1p, respectively. The family of longer chain dolichols appearing in the stationary growth phase contains dolichols with even more than 50 isoprene units exhibiting monotonically decreasing amounts with increasing chain lengths. The family of shorter chain dolichols is characteristic of the Rer2p products synthesized during the logarithmic growth phase of yeast. It forms a well-defined family of dolichols with 1318 isoprene residues with a certain level of longer dolichols composed of up to 24 isoprene units	13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + 19 isopentenyl diphosphate	Saccharomyces cerevisiae	-	19 diphosphate + di-trans,poly-cis-docosaprenyl diphosphate	dominating polyprenol with 22 isoprene residues, Srt1p products reaching C290 indicate the failure of a strict bacterial-like chain length control	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P35196	-	-
Saccharomyces cerevisiae	Q03175	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate	Rer2p Z-prenyltransferase synthesizes a well-defined family of polyprenols of 1318 isoprene residues with dominating C80 (16 isoprene residues) extending to C120. Two polyisoprenoid families with dominating C80 and C110 indicating the activity of both yeast Z-prenyltransferases Rer2p and Srt1p, respectively. The family of longer chain dolichols appearing in the stationary growth phase contains dolichols with even more than 50 isoprene units exhibiting monotonically decreasing amounts with increasing chain lengths. The family of shorter chain dolichols is characteristic of the Rer2p products synthesized during the logarithmic growth phase of yeast. It forms a well-defined family of dolichols with 1318 isoprene residues with a certain level of longer dolichols composed of up to 24 isoprene units	Saccharomyces cerevisiae	13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + 13 isopentenyl diphosphate	Rer2p Z-prenyltransferase synthesizes a well-defined family of polyprenols of 1318 isoprene residues with dominating C80 (16 isoprene residues) extending to C120	Saccharomyces cerevisiae	13 diphosphate + di-trans,poly-cis-hexadecaprenyl diphosphate	-	?
(2E,6E)-farnesyl diphosphate + 19 isopentenyl diphosphate	-	Saccharomyces cerevisiae	19 diphosphate + di-trans,poly-cis-docosaprenyl diphosphate	dominating polyprenol with 22 isoprene residues, Srt1p products reaching C290 indicate the failure of a strict bacterial-like chain length control	?

Synonyms

Synonyms	Comment	Organism
Rer2p	-	Saccharomyces cerevisiae
Rer2p Z-prenyltransferase	-	Saccharomyces cerevisiae
Srt1p	-	Saccharomyces cerevisiae
Srt2p Z-prenyltransferase	-	Saccharomyces cerevisiae

Expression

Organism	Comment	Expression
Saccharomyces cerevisiae	Rer2p is active in logarithmic phase, with dominating polyprenol with 16 isoprene residues	up
Saccharomyces cerevisiae	Srt1p is expressed in stationary phase with dominating polyprenol with 22 isoprene residues	up