Literature summary for 2.5.1.86 extracted from

Chan, H.C.; Feng, X.; Ko, T.P.; Huang, C.H.; Hu, Y.; Zheng, Y.; Bogue, S.; Nakano, C.; Hoshino, T.; Zhang, L.; Lv, P.; Liu, W.; Crick, D.C.; Liang, P.H.; Wang, A.H.; Oldfield, E.; Guo, R.T.
Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase from Mycobacterium tuberculosis (2014), J. Am. Chem. Soc., 136, 2892-2896.

Search for more literature for 2.5.1.86

Application

Application	Comment	Organism
drug development	the enzyme is a target for development of specific inhibitors for anti-infective therapies that block virulence factor formation, the enzyme is a target in treatment of tuberculosis	Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment)	Organism
gene Rv2361c, recombinant expression	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with BPH-640, hanging drop vapor diffusion method, using	Mycobacterium tuberculosis
purified recombinant enzyme in complex with inhibitor BPH-640, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
BPH-640	a bisphosphonate inhibitor, three-dimensional structure from crystal structure, dimeric DPPS structure with one bound BPH-640 molecule in each monomer, overview. BPH-640 occupies a dimer interface binding site, sandwiched between residues G77, N78, G79, R80, T83, R89, and R127 of monomer A and residues R292 and F293 of monomer B	Mycobacterium tuberculosis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	-	Mycobacterium tuberculosis	5618	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	Mycobacterium tuberculosis	-	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	Mycobacterium tuberculosis H37Rv	-	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate	Mycobacterium tuberculosis	-	diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate	Mycobacterium tuberculosis H37Rv	-	diphosphate + trans,octacis-decaprenyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WFF7	-	-
Mycobacterium tuberculosis	P9WFF7	gene Rv2361c	-
Mycobacterium tuberculosis H37Rv	P9WFF7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	-	Mycobacterium tuberculosis	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	the enzyme is a cis-prenyl transferase	Mycobacterium tuberculosis	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	-	Mycobacterium tuberculosis H37Rv	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate	the enzyme is a cis-prenyl transferase	Mycobacterium tuberculosis H37Rv	7 diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate	-	Mycobacterium tuberculosis	diphosphate + trans,octacis-decaprenyl diphosphate	-	?
(2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate	-	Mycobacterium tuberculosis H37Rv	diphosphate + trans,octacis-decaprenyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
cis-decaprenyl diphosphate synthase	-	Mycobacterium tuberculosis
decaprenyl diphosphate synthase	-	Mycobacterium tuberculosis
DPPS	-	Mycobacterium tuberculosis
Rv2361c	-	Mycobacterium tuberculosis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00041	-	pH and temperature not specified in the publication	Mycobacterium tuberculosis	BPH-640

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in cell wall biosynthesis	Mycobacterium tuberculosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)