Literature summary for 2.5.1.85 extracted from

Lai, D.H.; Poropat, E.; Pravia, C.; Landoni, M.; Couto, A.S.; Rojo, F.G.; Fuchs, A.G.; Dubin, M.; Elingold, I.; Rodriguez, J.B.; Ferella, M.; Esteva, M.I.; Bontempi, E.J.; Lukes, J.
Solanesyl diphosphate synthase, an enzyme of the ubiquinone synthetic pathway, is required throughout the life cycle of Trypanosoma brucei (2014), Eukaryot. Cell, 13, 320-328.

Search for more literature for 2.5.1.85

Protein Variants

Protein Variants	Comment	Organism
additional information	inhibition of TbSPPS expression by RNAi, inducible by tetracycline, causes effects of TbSPPS RNAi onmRNAand protein levels in the procyclic stage, viability of the BSF cells is also compromised upon RNAi induction, phenotype	Trypanosoma brucei

Inhibitors

Inhibitors	Comment	Organism	Structure
1-[(n-oct-1-ylamino)ethyl] 1,1-bisphosphonic acid	strong enzyme inhibitor	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39200	-	x * 39200, about, sequence calculation	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q38EX7	strains 29-13 and TREU927/4, single gene Tb09.160.4300, identical in both strains	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
bloodstream form	-	Trypanosoma brucei	-
additional information	in the parental 29-13 cells, enzyme TbSPPS is abundantly transcribed	Trypanosoma brucei	-

Subunits

Subunits	Comment	Organism
?	x * 39200, about, sequence calculation	Trypanosoma brucei

Synonyms

Synonyms	Comment	Organism
long-chain solanesyl diphosphate synthase	-	Trypanosoma brucei
solanesyl diphosphate synthase	-	Trypanosoma brucei
TbSPPS	-	Trypanosoma brucei

pI Value

Organism	Comment	pI Value Maximum	pI Value
Trypanosoma brucei	sequence calculation	-	6.12

General Information

General Information	Comment	Organism
malfunction	RNAi-mediated depletion of TbSPPS leads to severe growth inhibition. Ablation of TbSPPS by RNAi will decrease the function of the glycerol-3-phosphate shuttle, which is cured by application of glycerol. The viability of the BSF cells is compromised upon RNAi induction, phenotype overview. Addition of ubiquinone to the medium alleviates the effect of RNAi-mediated depletion of TbSPPS	Trypanosoma brucei
physiological function	solanesyl diphosphate synthase is an enzyme of the ubiquinone synthetic pathway required throughout the life cycle of Trypanosoma brucei	Trypanosoma brucei

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)