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Literature summary for 2.5.1.84 extracted from
- Nonaprenylpyrophosphate synthetase from Micrococcus luteus (1985), Methods Enzymol., 110, 206-209.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | 0.03-0.06%, 2fold stimulation | Micrococcus luteus |  |
| Tween 80 | 0.05-0.1%, 2fold stimulation | Micrococcus luteus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
all-trans-farnesyl diphosphate | pH 7.4, 37°C | Micrococcus luteus | |
| 0.00075 | - |
geranyl diphosphate | pH 7.4, 37°C | Micrococcus luteus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ is absolutely required for activity, Mn2+ is less effective. The chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and c40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds | Micrococcus luteus | |
| Mn2+ | Mg2+ is absolutely required for activity, Mn2+ is less effective | Micrococcus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
gel filtration | Micrococcus luteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Micrococcus luteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000787 | - |
- |
Micrococcus luteus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 2 weeks | Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| all-trans-farnesyl diphosphate + 6 isopentenyl diphosphate | - |
Micrococcus luteus | 6 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
| geranyl diphosphate + 7 isopentenyl diphosphate | - |
Micrococcus luteus | 7 diphosphate + all-trans-nonaprenyl diphosphate | the chain length of the synthesized polyprenyl diphosphate is dependent on the concentration of Mg2+ in the medium. At 0.1 mM Mg2+, C25, C30 and C35 compounds are produced with C25 predominating. At 0.5 mM Mg2+, C25, C30, C35 and C40 compounds are produced with C35 and C40 predominating. At 20 mM Mg2+, C40 and C45 are produced without accumulation od shorter compounds | ? | |
| geranylgeranyl diphosphate + 5 isopentenyl diphosphate | - |
Micrococcus luteus | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
| additional information | no activity with geranylneryl diphosphate or dimethylallyl diphosphate | Micrococcus luteus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nonaprenylpyrophosphate synthetase | - |
Micrococcus luteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Micrococcus luteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | in Tris-HCl buffer | Micrococcus luteus |
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Release 2023.1 (January 2023)
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