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Literature summary for 2.5.1.81 extracted from
- A geranylfarnesyl diphosphate synthase provides the precursor for sesterterpenoid (C25) formation in the glandular trichomes of the mint species Leucosceptrum canum (2016), Plant Cell, 28, 804-822.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Tween 20 | highly activating | Leucosceptrum canum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Lc-GFDPS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression in Escherichia coli, and in Arabidopsis thaliana driven by a double CaMV 35S promoter | Leucosceptrum canum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00165 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
| 0.00304 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Leucosceptrum canum | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by Mg2+ and Mn2+ | Leucosceptrum canum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate + isopentenyl diphosphate | Leucosceptrum canum | - |
(2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leucosceptrum canum | - |
gene Lc-GFDPS | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| glandular trichome | high expression level | Leucosceptrum canum | - |
| leaf | - |
Leucosceptrum canum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| geranylgeranyl diphosphate + isopentenyl diphosphate | - |
Leucosceptrum canum | (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GFDP synthase | - |
Leucosceptrum canum |
| GFDPS | - |
Leucosceptrum canum |
| IDS3 | - |
Leucosceptrum canum |
| Lc-GFDPS | - |
Leucosceptrum canum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Leucosceptrum canum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000517 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
| 0.0000082 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Leucosceptrum canum |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Leucosceptrum canum | application of salicilic acid and methyl jasmonate induce GFDPS transcript and sesterterpenoid accumulation | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | phylogenetic analysis suggest that GFDPS probably evolved from plant geranylgeranyl diphosphate synthase under the influence of positive selection. Lc-GFDPS clusters in subcluster Ia, which is dominated by GGDPS sequences | Leucosceptrum canum |
| metabolism | biosynthesis of the C25 precursor geranylfarnesyl diphosphate to leucosceptroid sesterterpenoids is catalyzed by enzyme GFDPS that uses substrates geranylgeranyl diphosphate and Iisopentenyl diphosphate derived from the plastidial MEP pathway, overview | Leucosceptrum canum |
| physiological function | the geranylfarnesyl diphosphate synthase provides the precursor for sesterterpenoid (C25) formation in the glandular trichomes of the mint species Leucosceptrum canum, the C25 terpenoids might play a defensive role | Leucosceptrum canum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
isopentenyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
| 0.003 | - |
geranylgeranyl diphosphate | pH 7.5, 30°C | Leucosceptrum canum | |
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