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Literature summary for 2.5.1.78 extracted from

  • Azuma, Y.; Zschoche, R.; Hilvert, D.
    The C-terminal peptide of Aquifex aeolicus riboflavin synthase directs encapsulation of native and foreign guests by a cage-forming lumazine synthase (2017), J. Biol. Chem., 292, 10321-10327 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.78

Cloned(Commentary)

Cloned (Comment) Organism
lumazine synthase forms cage complexes with the cognate riboflavin synthase from Aquifex aeolicus when both proteins are co-produced in the cytosol of Escherichia coli. A 12-amino acid-long peptide at the C terminus of riboflavin synthase serves as a specific localization sequence responsible for targeting the guest to the protein compartment Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
additional information a 12-amino acid-long peptide at the C terminus of riboflavin synthase serves as a specific localization sequence responsible for targeting the guest to the protein compartment. Covalent fusion of this peptide tag to heterologous guest molecules leads to their internalization into lumazine synthase assemblies both in vivo and in vitro Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil Aquifex aeolicus
-
 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-
Aquifex aeolicus O66529
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil
-
 Aquifex aeolicus 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
lumazine synthase
-
 Aquifex aeolicus

General Information

General Information Comment Organism
metabolism lumazine synthase forms specific inclusion complexes with riboflavin synthase when both proteins are co-expressed in a heterologous host. Encapsulation of specific enzymes in self-assembling protein cages is a hallmark of bacterial compartments that function as counterparts to eukaryotic organelles Aquifex aeolicus
physiological function lumazine synthase forms cage complexes with the cognate riboflavin synthase when both proteins are coproduced in the cytosol of Escherichia coli. A 12-amino acid-long peptide at the C terminus of riboflavin synthase serves as a specific localization sequence responsible for targeting the guest to the protein compartment Aquifex aeolicus