Literature summary for 2.5.1.78 extracted from

Du, Z.Q.; Wang, J.Y.
A novel lumazine synthase molecule from Brucella significantly promotes the immune-stimulation effects of antigenic protein (2015), Genet. Mol. Res., 14, 13084-13095.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene bls, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of enzyme BLS fused to protein omp31 as His-tagged protein in Escherichia coli strain BL21(DE3)	Brucella suis

Organism

Organism	UniProt	Comment	Textmining
Brucella suis	P61711	gene bls	-

Purification (Commentary)

Purification (Comment)	Organism
gene bls, recombinant GST-tagged enzyme BLS and recombinant His-tagged BLS-Omp31 fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and nickel affinity chromatography, respectively, and tag removal using thrombin	Brucella suis

Subunits

Subunits	Comment	Organism
More	the recombinant enzyme polymerizes and exists in different forms, including as a dimer and pentamer, overview	Brucella suis

Synonyms

Synonyms	Comment	Organism
BLS	-	Brucella suis
DMRL synthase 2	UniProt	Brucella suis
lumazine synthase	-	Brucella suis
lumazine synthase 2	UniProt	Brucella suis
type II lumazine synthase	UniProt	Brucella suis

General Information

General Information	Comment	Organism
physiological function	the lumazine synthase molecule from Brucella significantly promotes the immune-stimulation effects of antigenic proteins, e.g Omp31, overview	Brucella suis

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Release 2023.1 (January 2023)