Literature summary for 2.5.1.78 extracted from

Shankar, M.; Wilbanks, S.M.; Nakatani, Y.; Monk, B.C.; Tyndall, J.D.
Catalysis product captured in lumazine synthase from the fungal pathogen Candida glabrata (2013), Acta Crystallogr. Sect. D, 69, 1580-1586.

Application

Application	Comment	Organism
drug development	the enzyme is a potential drug target for the development of broad-spectrum antifungal drugs	[Candida] glabrata

Cloned(Commentary)

Cloned (Comment)	Organism
gene RIB4A, recombinant expression of His-tagged enzyme in Escherichia coli	[Candida] glabrata

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 200 nl 8.4 mg/ml protein in 100 mM potassium phosphate, pH 7.5, and 150 mM NaCl, with 200 nl of reservoir solution containing 1.6 M ammonium sulfate, 100 mM sodium acetate pH 5.5, 100 mM sodium chloride, and equilibration against 0.1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.24 A resolution	[Candida] glabrata

Crystallization (Comment)

Organism

purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 200 nl 8.4 mg/ml protein in 100 mM potassium phosphate, pH 7.5, and 150 mM NaCl, with 200 nl of reservoir solution containing 1.6 M ammonium sulfate, 100 mM sodium acetate pH 5.5, 100 mM sodium chloride, and equilibration against 0.1 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.24 A resolution

[Candida] glabrata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
97770	-	MALDI-TOF mass spectromety	[Candida] glabrata

Organism

Organism	UniProt	Comment	Textmining
[Candida] glabrata	Q6FXA8	gene RIB4A	-

Purification (Commentary)

Purification (Comment)	Organism
gene RIB4A, recombinant His-tagged enzyme from Escherichia coli by nickel affinityy chromatography, gel filtration and ultrafiltration	[Candida] glabrata

Subunits

Subunits	Comment	Organism
decamer	10 * 19548, His-tagged enzyme, (alpha5)2, a dimer of homopentamers with one in five subunits containing a product molecule from the catalytic reaction	[Candida] glabrata

Synonyms

Synonyms	Comment	Organism
6,7-dimethyl-8-(d-ribityl)lumazine synthase	-	[Candida] glabrata
lumazine synthase	-	[Candida] glabrata

General Information

General Information	Comment	Organism
physiological function	the enzyme 6,7-dimethyl-8-(d-ribityl)lumazine synthase is part of the riboflavin biosynthesis pathway essential to fungi and bacteria	[Candida] glabrata