Literature summary for 2.5.1.78 extracted from

Gerhardt, S.; Haase, I.; Steinbacher, S.; Kaiser, J.T.; Cushman, M.; Bacher, A.; Huber, R.; Fischer, M.
The structural basis of riboflavin binding to Schizosaccharomyces pombe 6,7-dimethyl-8-ribityllumazine synthase (2002), J. Mol. Biol., 318, 1317-1329.

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, the enzyme is crystallised either in complex with bound riboflavin (RIBO) or in complex with the substrate analogue 5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione (NRAP) or the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine (CEOL). The mutant proteins W27G, W63Y and W63Y/L119F, which do not bind riboflavin, and the mutant L119F, which only weakly binds to riboflavin, are also analysed. Diffraction data are collected to resolutions of 2.4 A (RIBO), 2.4 A (NRAP), 2.6 A (CEOL), 2.0 A (W27G), 3.1 A (W63Y and L119F) and 2.7 A (W63Y/L119F), respectively. All crystals belong to space group C222(1) with one pentamer in the asymmetric unit corresponding to the solution state of the protein	Schizosaccharomyces pombe

Crystallization (Comment)

Organism

sitting drop vapour diffusion method, the enzyme is crystallised either in complex with bound riboflavin (RIBO) or in complex with the substrate analogue 5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione (NRAP) or the product analogue 6-carboxyethyl-7-oxo-8-ribityllumazine (CEOL). The mutant proteins W27G, W63Y and W63Y/L119F, which do not bind riboflavin, and the mutant L119F, which only weakly binds to riboflavin, are also analysed. Diffraction data are collected to resolutions of 2.4 A (RIBO), 2.4 A (NRAP), 2.6 A (CEOL), 2.0 A (W27G), 3.1 A (W63Y and L119F) and 2.7 A (W63Y/L119F), respectively. All crystals belong to space group C222(1) with one pentamer in the asymmetric unit corresponding to the solution state of the protein

Schizosaccharomyces pombe

Protein Variants

Protein Variants	Comment	Organism
L119F	weakly binds to riboflavin	Schizosaccharomyces pombe
W27G	does not bind riboflavin	Schizosaccharomyces pombe
W63Y	does not bind riboflavin	Schizosaccharomyces pombe
W63Y/L119F	does not bind riboflavin	Schizosaccharomyces pombe

Inhibitors

Inhibitors	Comment	Organism	Structure
5-nitro-6-(D-ribitylamino)-2,4(1H,3H)-pyrimidinedione	-	Schizosaccharomyces pombe
6-carboxyethyl-7-oxo-8-ribityllumazine	-	Schizosaccharomyces pombe

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Schizosaccharomyces pombe

Subunits

Subunits	Comment	Organism
pentamer	-	Schizosaccharomyces pombe