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Literature summary for 2.5.1.78 extracted from
- Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase (1999), J. Biol. Chem., 274, 22114-22121.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Spinacia oleracea |
| expression in Escherichia coli | Nicotiana tabacum |
| expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | - |
Spinacia oleracea |  |
| 0.026 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | - |
Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | constitutes less than 0.02% of the total chloroplast protein. The enzyme is exclusivelylocated in the chloroplast stroma | Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15500 | - |
60 * 15500, subunit of mature enzyme, SDS-PAGE | Spinacia oleracea |
| 16534 | - |
60 * 16534, subunit of mature enzyme, calculated from sequence | Spinacia oleracea |
| 16536 | - |
60 * 16536, subunit of mature enzyme, electrospray ionization mass spectrometry | Spinacia oleracea |
| 850000 | - |
gel filtration | Spinacia oleracea |
| 973000 | 991000 | sedimentaion equilibrium studies | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Spinacia oleracea | lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Nicotiana tabacum | lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Arabidopsis thaliana | penultimate step of riboflavin biosynthesis | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Nicotiana tabacum | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | plant lumazine synthase is synthesized in the cytosol as a larger molecular weight precursor protein, which is post-translationally imported into chloroplasts where it is proteolytically cleaved to its mature size | Spinacia oleracea |
| proteolytic modification | plant lumazine synthase is synthesized in the cytosol as a larger molecular weight precursor protein, which is post-translationally imported into chloroplasts where it is proteolytically cleaved to its mature size | Nicotiana tabacum |
| proteolytic modification | plant lumazine synthase is synthesized in the cytosol as a larger molecular weight precursor protein, which is post-translationally imported into chloroplasts where it is proteolytically cleaved to its mature size | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.5 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Spinacia oleracea | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Nicotiana tabacum | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Arabidopsis thaliana | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis | Spinacia oleracea | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | lumazine synthase catalyzes the penultimate step of riboflavin biosynthesis | Nicotiana tabacum | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
| 5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | penultimate step of riboflavin biosynthesis | Arabidopsis thaliana | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| 60-mer | 60 * 15500, subunit of mature enzyme, SDS-PAGE | Spinacia oleracea |
| 60-mer | 60 * 16534, subunit of mature enzyme, calculated from sequence | Spinacia oleracea |
| 60-mer | 60 * 16536, subunit of mature enzyme, electrospray ionization mass spectrometry | Spinacia oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lumazine synthase | - |
Spinacia oleracea |
| lumazine synthase | - |
Nicotiana tabacum |
| lumazine synthase | - |
Arabidopsis thaliana |
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