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Literature summary for 2.5.1.78 extracted from

  • Mörtl, S.; Fischer, M.; Richter, G.; Tack, J.; Weinkauf, S.; Bacher, A.
    Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli (1996), J. Biol. Chem., 271, 33201-33207.
    View publication on PubMed
Search for more literature for 2.5.1.78

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione
-
 Saccharomyces cerevisiae
0.0042
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione
-
 Escherichia coli
0.062
-
 L-3,4-dihydroxybutan-2-one 4-phosphate
-
 Escherichia coli
0.09
-
 L-3,4-dihydroxybutan-2-one 4-phosphate
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 the protein is an icosahedral capsid of 60 subunits with a mass of about 1 MDa as shown by hydrodynamic studies and by electron microscopy. The lumazine synthase of Escherichia coli is not physically associated with another enzyme of the riboflavin pathway, and the core of the icosahedral capsid is empty Escherichia coli
16156
-
 60 * 16156, calculated from sequence Escherichia coli
18598
-
 5 * 18598, calculated from sequence Saccharomyces cerevisiae
90000
-
 sedimentation equilibrium analysis Saccharomyces cerevisiae
977000
-
 sedimentation equilibrium analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Escherichia coli the enzyme is involved in riboflavin biosynthesis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Saccharomyces cerevisiae the enzyme is involved in riboflavin biosynthesis 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Escherichia coli
recombinant Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.8
-
-
 Escherichia coli
15.4
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Escherichia coli 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Saccharomyces cerevisiae 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate the enzyme is involved in riboflavin biosynthesis Escherichia coli 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate the enzyme is involved in riboflavin biosynthesis Saccharomyces cerevisiae 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
60-mer 60 * 16156, calculated from sequence Escherichia coli
pentamer 5 * 18598, calculated from sequence Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
ribE
-
 Escherichia coli