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Literature summary for 2.5.1.78 extracted from

  • Zylberman, V.; Klinke, S.; Haase, I.; Bacher, A.; Fischer, M.; Goldbaum, F.A.
    Evolution of vitamin B2 biosynthesis: 6,7-dimethyl-8-ribityllumazine synthases of Brucella (2006), J. Bacteriol., 188, 6135-6142.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.78

Cloned(Commentary)

Cloned (Comment) Organism
-
 Brucella sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione
-
 Brucella sp.
0.125
-
 L-3,4-dihydroxybutan-2-one 4-phosphate
-
 Brucella sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17599
-
 5 * 17599, calculated from sequence Brucella sp.
88000
-
 sedimentation equilibrium experiments Brucella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate Brucella sp. penultimate step in the biosynthesis of riboflavin. The type II lumazine synthase is an immunodominant antigen of Brucella abortus 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Brucella sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brucella sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.018
-
-
 Brucella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate
-
 Brucella sp. 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate penultimate step in the biosynthesis of riboflavin. The type II lumazine synthase is an immunodominant antigen of Brucella abortus Brucella sp. 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 17599, calculated from sequence Brucella sp.

Synonyms

Synonyms Comment Organism
ribH1
-
 Brucella sp.
RibH1 protein
-
 Brucella sp.
type I lumazine synthase
-
 Brucella sp.