Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | 37°C, pH 7.0 | Aquifex aeolicus | |
0.0125 | - |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione | 37°C, pH 7.0 | Methanocaldococcus jannaschii | |
0.026 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | 37°C, pH 7.0 | Aquifex aeolicus | |
0.052 | - |
L-3,4-dihydroxybutan-2-one 4-phosphate | 37°C, pH 7.0 | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15645 | - |
60 * 15645, the enzyme can form capsids with icosahedral 532 symmetry consisting of 60 subunits, electrospray mass spectrometry | Methanocaldococcus jannaschii |
16000 | - |
60 * 16000, the enzyme can form capsids with icosahedral 532 symmetry consisting of 60 subunits, SDS-PAGE | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Aquifex aeolicus | the enzyme is involved in biosynthesis of riboflavin | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | Methanocaldococcus jannaschii | the enzyme is involved in biosynthesis of riboflavin | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Methanocaldococcus jannaschii | Q57751 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
recombinant enzyme | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
37°C, pH 7.0 | Methanocaldococcus jannaschii |
0.031 | - |
37°C, pH 7.0 | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Aquifex aeolicus | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | - |
Methanocaldococcus jannaschii | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | the enzyme is involved in biosynthesis of riboflavin | Aquifex aeolicus | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-(1-D-ribitylamino)pyrimidine-2,4(1H,3H)-dione + (S)-2-hydroxy-3-oxobutyl dihydrogen phosphate | the enzyme is involved in biosynthesis of riboflavin | Methanocaldococcus jannaschii | 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O | - |
? | |
5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
Aquifex aeolicus | 6,7-dimethyl-8-(1-D-ribityl)lumazine + 2 H2O + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
60-mer | 60 * 15645, the enzyme can form capsids with icosahedral 532 symmetry consisting of 60 subunits, electrospray mass spectrometry | Methanocaldococcus jannaschii |
60-mer | 60 * 16000, the enzyme can form capsids with icosahedral 532 symmetry consisting of 60 subunits, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
MJ0303 | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aquifex aeolicus |
37 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aquifex aeolicus |
7 | - |
assay at | Methanocaldococcus jannaschii |