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Literature summary for 2.5.1.75 extracted from

  • Soderberg, T.; Poulter, C.D.
    Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: site-directed mutagenesis of highly conserved residues (2001), Biochemistry, 40, 1734-1740.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D153A at least 25% of wild-type activity Escherichia coli
D164A increase in Km for both substrates Escherichia coli
D42A 20fold decrease in kcat Escherichia coli
E173A at least 25% of wild-type activity Escherichia coli
E229A substantial increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations Escherichia coli
F84A at least 25% of wild-type activity Escherichia coli
H67F 25fold increase in Km for dimethylallyl diphosphate Escherichia coli
K23A increase in Km for both substrates Escherichia coli
K280A increase in Km for both substrates Escherichia coli
K56A 15fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations Escherichia coli
Q253A 18fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations Escherichia coli
Q282A increase in both Km and kcat value Escherichia coli
R167A increase in Km for both substrates Escherichia coli
R170A increase in Km for both substrates Escherichia coli
R213A at least 25% of wild-type activity Escherichia coli
R217A increase in Km and decrease in kcat for dimethylallyl diphosphate Escherichia coli
R281A at least 25% of wild-type activity Escherichia coli
S178A at least 25% of wild-type activity Escherichia coli
S43A at least 25% of wild-type activity Escherichia coli
T108A increase in Km for both substrates Escherichia coli
T19A about 600fold decrease in kcat Escherichia coli
T24A increase in Km for both substrates Escherichia coli
T275A at least 25% of wild-type activity Escherichia coli
T54A increase in Km for both substrates Escherichia coli
W285A at least 25% of wild-type activity Escherichia coli
Y111F at least 25% of wild-type activity Escherichia coli
Y111S at least 25% of wild-type activity Escherichia coli
Y47F at least 25% of wild-type activity Escherichia coli
Y47S 100fold decrease in kcat Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
dimethylallyl diphosphate mutant Y47S, pH 8.0 Escherichia coli
1.5
-
seventeen-base RNA oligonucleotide mutant H67F, pH 8.0 Escherichia coli
2.2
-
dimethylallyl diphosphate wild-type, pH 8.0 Escherichia coli
2.3
-
dimethylallyl diphosphate mutant Q253A, pH 8.0 Escherichia coli
2.8
-
seventeen-base RNA oligonucleotide mutant D42A, pH 8.0 Escherichia coli
2.9
-
dimethylallyl diphosphate mutant D42A, pH 8.0 Escherichia coli
3.2
-
seventeen-base RNA oligonucleotide wild-type, pH 8.0 Escherichia coli
3.5
-
dimethylallyl diphosphate mutant Y47F, pH 8.0 Escherichia coli
4.4
-
seventeen-base RNA oligonucleotide mutant Y47F, pH 8.0 Escherichia coli
7.2
-
seventeen-base RNA oligonucleotide mutant R217A, pH 8.0 Escherichia coli
8.9
-
seventeen-base RNA oligonucleotide mutant Y47S, pH 8.0 Escherichia coli
10
-
dimethylallyl diphosphate mutant K56A, pH 8.0 Escherichia coli
12
-
seventeen-base RNA oligonucleotide mutant T54A, pH 8.0 Escherichia coli
13
-
dimethylallyl diphosphate mutant T54A, pH 8.0 Escherichia coli
16
-
seventeen-base RNA oligonucleotide mutant T108A, pH 8.0 Escherichia coli
22
-
dimethylallyl diphosphate mutant T108A, pH 8.0 Escherichia coli
27
-
dimethylallyl diphosphate mutant d164A, pH 8.0 Escherichia coli
27
-
seventeen-base RNA oligonucleotide mutant d164A, pH 8.0 Escherichia coli
45
-
seventeen-base RNA oligonucleotide mutant Q282A, pH 8.0 Escherichia coli
50
-
dimethylallyl diphosphate mutant H67F, pH 8.0 Escherichia coli
50
-
seventeen-base RNA oligonucleotide mutant K56A, pH 8.0 Escherichia coli
56
-
seventeen-base RNA oligonucleotide mutant Q253A, pH 8.0 Escherichia coli
60
-
dimethylallyl diphosphate mutant Q282A, pH 8.0 Escherichia coli
75
-
seventeen-base RNA oligonucleotide mutant R167A, pH 8.0 Escherichia coli
114
-
dimethylallyl diphosphate mutant R217A, pH 8.0 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + seventeen-base RNA oligonucleotide sequence 5'GCGGACUCAAAAUCCGC3', RNA oligonucleotide based on the unmodified stem-loop region of tRNAPhe Escherichia coli diphosphate + seventeen-base RNA oligonucleotide containing 6-dimethylallyladenosine
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
seventeen-base RNA oligonucleotide mutant Y47S, pH 8.0 Escherichia coli
0.007
-
seventeen-base RNA oligonucleotide mutant R217A, pH 8.0 Escherichia coli
0.009
-
seventeen-base RNA oligonucleotide mutant D42A, pH 8.0 Escherichia coli
0.07
-
seventeen-base RNA oligonucleotide mutant T108A, pH 8.0 Escherichia coli
0.07
-
seventeen-base RNA oligonucleotide mutant T54A, pH 8.0 Escherichia coli
0.15
-
seventeen-base RNA oligonucleotide mutant D164A, pH 8.0 Escherichia coli
0.18
-
seventeen-base RNA oligonucleotide mutant H67F, pH 8.0 Escherichia coli
0.19
-
seventeen-base RNA oligonucleotide wild-type, pH 8.0 Escherichia coli
0.23
-
seventeen-base RNA oligonucleotide mutant Y47F, pH 8.0 Escherichia coli
0.3
-
seventeen-base RNA oligonucleotide mutnat Q282A, pH 8.0 Escherichia coli