Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.75 extracted from

  • Rosenbaum, N.; Gefter, M.L.
    DELTA 2 -isopentenylpyrophosphate: transfer ribonucleic acid 2 -isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme (1972), J. Biol. Chem., 247, 5675-5680.
    View publication on PubMed

General Stability

General Stability Organism
not stable to ammonium sulfate precipitation Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
Escherichia coli
EDTA
-
Escherichia coli
high ionic strength
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ 3.3 mM Escherichia coli
Mn2+ can partially replace Mg2+ in activation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.053
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
0°C, stable for 1 month Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + adenine37 in tRNA tRNA lacking the isopentenyl modification normally present in vivo Escherichia coli diphosphate + N6-dimethylallyladenine37 in tRNA
-
?
dimethylallyl diphosphate + adenine37 in tRNA specific for DELTA2-isopentenyl phosphate Escherichia coli diphosphate + N6-dimethylallyladenine37 in tRNA
-
?
dimethylallyl diphosphate + adenine37 in tRNA no reaction with DELTA3-isopentenyl diphosphate Escherichia coli diphosphate + N6-dimethylallyladenine37 in tRNA
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 50% of maximum activity at pH 6 and pH 9, below pH 5 activity is totally abolished Escherichia coli