General Stability | Organism |
---|---|
not stable to ammonium sulfate precipitation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Escherichia coli | |
EDTA | - |
Escherichia coli | |
high ionic strength | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | 3.3 mM | Escherichia coli | |
Mn2+ | can partially replace Mg2+ in activation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.053 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
0°C, stable for 1 month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + adenine37 in tRNA | tRNA lacking the isopentenyl modification normally present in vivo | Escherichia coli | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNA | specific for DELTA2-isopentenyl phosphate | Escherichia coli | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
dimethylallyl diphosphate + adenine37 in tRNA | no reaction with DELTA3-isopentenyl diphosphate | Escherichia coli | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 50% of maximum activity at pH 6 and pH 9, below pH 5 activity is totally abolished | Escherichia coli |