BRENDA - Enzyme Database
show all sequences of 2.5.1.73

Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei

Hauenstein, S.I.; Perona, J.J.; J. Biol. Chem. 283, 22007-22017 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta2(DE3) pLysS cells; expression in Escherichia coli
Methanosarcina mazei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
2 * 42000, gel filtration
Methanosarcina mazei
42324
-
2 * 42324, predicted from amino acid sequence
Methanosarcina mazei
84000
-
gel filtration
Methanosarcina mazei
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina mazei
-
-
-
Methanosarcina mazei
Q8PVS9
-
-
Purification (Commentary)
Commentary
Organism
; DEAE column chromatography and Superdex 200 gel filtration
Methanosarcina mazei
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
highly sensitive assay to measure the activity of SepCysS under anaerobic conditions
Methanosarcina mazei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
thiosulfate shows 72.6% of the activity compared to sulfide as sulfur donor, cysteine shows 77% of the activity compared to sulfide as sulfur donor. No correct Cys-tRNACys product is formed in presence of these sulfur donors
687743
Methanosarcina mazei
?
-
-
-
-
O-phospho-L-seryl-tRNACys + sulfide
under steady-state conditions at 1-50 nM Sep-CysS, there is no reaction when O-phospho-L-seryl-tRNACys substrate levels are maintained below 0.01 mM, however, product formation is detected at 0.05 mM O-phospho-L-seryl-tRNACys
687743
Methanosarcina mazei
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
SepCysS exhibits substrate preference among the three Methanosarcina mazei tRNACys isoacceptors
687743
Methanosarcina mazei
L-cysteinyl-tRNACys + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 42000, gel filtration; 2 * 42324, predicted from amino acid sequence
Methanosarcina mazei
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
both active sites bind the cofactor; dependent
Methanosarcina mazei
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta2(DE3) pLysS cells
Methanosarcina mazei
expression in Escherichia coli
Methanosarcina mazei
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
both active sites bind the cofactor
Methanosarcina mazei
pyridoxal 5'-phosphate
dependent
Methanosarcina mazei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
2 * 42000, gel filtration
Methanosarcina mazei
42324
-
2 * 42324, predicted from amino acid sequence
Methanosarcina mazei
84000
-
gel filtration
Methanosarcina mazei
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanosarcina mazei
DEAE column chromatography and Superdex 200 gel filtration
Methanosarcina mazei
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
highly sensitive assay to measure the activity of SepCysS under anaerobic conditions
Methanosarcina mazei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
thiosulfate shows 72.6% of the activity compared to sulfide as sulfur donor, cysteine shows 77% of the activity compared to sulfide as sulfur donor. No correct Cys-tRNACys product is formed in presence of these sulfur donors
687743
Methanosarcina mazei
?
-
-
-
-
O-phospho-L-seryl-tRNACys + sulfide
under steady-state conditions at 1-50 nM Sep-CysS, there is no reaction when O-phospho-L-seryl-tRNACys substrate levels are maintained below 0.01 mM, however, product formation is detected at 0.05 mM O-phospho-L-seryl-tRNACys
687743
Methanosarcina mazei
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
SepCysS exhibits substrate preference among the three Methanosarcina mazei tRNACys isoacceptors
687743
Methanosarcina mazei
L-cysteinyl-tRNACys + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 42000, gel filtration; 2 * 42324, predicted from amino acid sequence
Methanosarcina mazei
Other publictions for EC 2.5.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728697
Liu
Ancient translation factor is ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Proc. Natl. Acad. Sci. USA
111
10520-10505
2014
1
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1
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722286
Helgadottir
Mutational analysis of Sep-tRN ...
Methanocaldococcus jannaschii
FEBS Lett.
586
60-63
2012
-
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19
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3
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722799
Liu
Catalytic mechanism of Sep-tRN ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Biol. Chem.
287
5426-5433
2012
-
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1
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6
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6
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1
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4
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1
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6
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1
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4
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1
1
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722261
Yuan
A tRNA-dependent cysteine bios ...
Escherichia coli
FEBS Lett.
584
2857-2861
2010
-
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2
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684126
Yuan
Amino acid modifications on tR ...
Archaeoglobus fulgidus, Methanococcus maripaludis, no activity in Methanobrevibacter smithii, no activity in Methanosphaera stadtmanae
Acta Biochim. Biophys. Sin. (Shanghai)
40
539-553
2008
-
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1
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1
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5
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4
1
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2
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3
2
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1
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4
1
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687743
Hauenstein
Redundant synthesis of cystein ...
Methanosarcina mazei
J. Biol. Chem.
283
22007-22017
2008
-
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1
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-
-
-
-
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3
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4
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1
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1
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3
1
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1
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2
2
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3
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2
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1
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3
1
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-
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-
-
-
-
689145
Zhang
Aminoacylation of tRNA with ph ...
Methanocaldococcus jannaschii
Nat. Struct. Mol. Biol.
15
507-514
2008
-
-
1
-
-
-
-
-
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1
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3
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1
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3
1
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1
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1
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1
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3
1
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675447
Fukunaga
Structural insights into the s ...
Archaeoglobus fulgidus
J. Mol. Biol.
370
128-141
2007
-
-
1
1
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-
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1
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2
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3
-
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1
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2
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1
1
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1
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4
2
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676784
O'Donoghue
The evolutionary history of Cy ...
Archaeoglobus fulgidus, Methanococcoides burtonii, Methanopyrus kandleri, Methanospirillum hungatei
Proc. Natl. Acad. Sci. USA
102
19003-19008
2005
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4
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4
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4
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8
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4
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4
4
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4
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8
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677118
Sauerwald
RNA-dependent cysteine biosynt ...
Methanocaldococcus jannaschii, Methanococcus maripaludis
Science
307
1969-1972
2005
-
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1
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1
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2
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5
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1
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4
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2
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1
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1
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2
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1
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4
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