BRENDA - Enzyme Database
show all sequences of 2.5.1.73

Amino acid modifications on tRNA

Yuan, J.; Sheppard, K.; Soell, D.; Acta Biochim. Biophys. Sin. (Shanghai) 40, 539-553 (2008)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
;
Archaeoglobus fulgidus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-seryl-tRNACys + sulfide
Methanococcus maripaludis
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential
L-cysteinyl-tRNACys + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Archaeoglobus fulgidus
-
-
-
Archaeoglobus fulgidus
O30207
-
-
Methanococcus maripaludis
-
-
-
no activity in Methanobrevibacter smithii
-
-
-
no activity in Methanosphaera stadtmanae
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-seryl-tRNACys + sulfide
-
684126
Archaeoglobus fulgidus
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential
684126
Methanococcus maripaludis
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient
684126
Archaeoglobus fulgidus
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient
684126
Methanococcus maripaludis
L-cysteinyl-tRNACys + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
x-ray crystallography
Archaeoglobus fulgidus
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
; dependent
Archaeoglobus fulgidus
pyridoxal 5'-phosphate
-
Methanococcus maripaludis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
dependent
Archaeoglobus fulgidus
pyridoxal 5'-phosphate
-
Archaeoglobus fulgidus
pyridoxal 5'-phosphate
-
Methanococcus maripaludis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Archaeoglobus fulgidus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-seryl-tRNACys + sulfide
Methanococcus maripaludis
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential
L-cysteinyl-tRNACys + phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-seryl-tRNACys + sulfide
-
684126
Archaeoglobus fulgidus
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient. Methanococcus maripaludis encodes both the direct and indirect paths for Cys-tRNACys synthesis. While sepS (encoding SepRS) can be deleted when the organism is grown in the presence of Cys, pscS (encoding SepCysS) cannot. SepCysS may possess an additional function in Methanococcus maripaludis that is essential
684126
Methanococcus maripaludis
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient
684126
Archaeoglobus fulgidus
L-cysteinyl-tRNACys + phosphate
-
-
-
?
O-phospho-L-seryl-tRNACys + sulfide
the sulfur donor for this enzyme is unknown though in vitro sulfide is sufficient
684126
Methanococcus maripaludis
L-cysteinyl-tRNACys + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
x-ray crystallography
Archaeoglobus fulgidus
Other publictions for EC 2.5.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728697
Liu
Ancient translation factor is ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Proc. Natl. Acad. Sci. USA
111
10520-10505
2014
1
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1
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5
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722286
Helgadottir
Mutational analysis of Sep-tRN ...
Methanocaldococcus jannaschii
FEBS Lett.
586
60-63
2012
-
-
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-
19
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3
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19
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722799
Liu
Catalytic mechanism of Sep-tRN ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
J. Biol. Chem.
287
5426-5433
2012
-
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1
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6
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6
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1
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4
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1
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6
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1
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4
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1
1
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-
722261
Yuan
A tRNA-dependent cysteine bios ...
Escherichia coli
FEBS Lett.
584
2857-2861
2010
-
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2
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2
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684126
Yuan
Amino acid modifications on tR ...
Archaeoglobus fulgidus, Methanococcus maripaludis, no activity in Methanobrevibacter smithii, no activity in Methanosphaera stadtmanae
Acta Biochim. Biophys. Sin. (Shanghai)
40
539-553
2008
-
-
-
1
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
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4
1
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-
-
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-
-
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2
-
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3
2
-
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1
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-
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-
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4
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
687743
Hauenstein
Redundant synthesis of cystein ...
Methanosarcina mazei
J. Biol. Chem.
283
22007-22017
2008
-
-
1
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-
-
-
-
-
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3
-
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4
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1
-
-
-
1
-
3
1
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-
-
-
-
-
1
-
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-
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2
2
-
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3
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-
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2
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
689145
Zhang
Aminoacylation of tRNA with ph ...
Methanocaldococcus jannaschii
Nat. Struct. Mol. Biol.
15
507-514
2008
-
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1
-
-
-
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1
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3
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1
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3
1
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1
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1
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1
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3
1
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-
-
-
-
-
-
-
-
-
-
-
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-
675447
Fukunaga
Structural insights into the s ...
Archaeoglobus fulgidus
J. Mol. Biol.
370
128-141
2007
-
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1
1
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-
-
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1
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2
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3
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1
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4
2
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1
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1
1
1
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1
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2
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1
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4
2
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-
676784
O'Donoghue
The evolutionary history of Cy ...
Archaeoglobus fulgidus, Methanococcoides burtonii, Methanopyrus kandleri, Methanospirillum hungatei
Proc. Natl. Acad. Sci. USA
102
19003-19008
2005
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4
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4
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4
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8
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4
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4
4
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4
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8
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-
-
-
-
-
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-
-
-
-
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-
677118
Sauerwald
RNA-dependent cysteine biosynt ...
Methanocaldococcus jannaschii, Methanococcus maripaludis
Science
307
1969-1972
2005
-
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1
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1
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-
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2
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5
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1
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4
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2
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1
2
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1
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2
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1
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4
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