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Literature summary for 2.5.1.71 extracted from
- Characterization of leachianone G 2''-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures (2003), Plant Physiol., 133, 1306-1313.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
leachianone G | pH 8.8, 30°C | Sophora flavescens |  |
| 0.059 | - |
dimethylallyl diphosphate | pH 8.8, 30°C | Sophora flavescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Sophora flavescens | 9507 | - |
| membrane | bound, determination of enzyme distibution in membranes | Sophora flavescens | 16020 | - |
| additional information | subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview | Sophora flavescens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 25% of the activity with Mg2+ | Sophora flavescens | |
| Co2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
| Cu2+ | 4% of the activity with Mg2+ | Sophora flavescens | |
| Fe2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
| Mg2+ | required, optimal at 10 mM, other divalent cations can only partially substitute for Mg2+, overview | Sophora flavescens | |
| Mn2+ | 15% of the activity with Mg2+ | Sophora flavescens | |
| Ni2+ | 5% of the activity with Mg2+ | Sophora flavescens | |
| Zn2+ | 10% of the activity with Mg2+ | Sophora flavescens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + leachianone G | Sophora flavescens | the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant | diphosphate + sophoraflavanone G | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sophora flavescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by microsome preparation | Sophora flavescens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dimethylallyl diphosphate + leachianone G = diphosphate + sophoraflavanone G | reaction mechanism | Sophora flavescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Sophora flavescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00014 | - |
crude cell extract | Sophora flavescens |
| 0.06 | - |
microsomes | Sophora flavescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + euchrenone a7 | 54% of the activity with leachianone G | Sophora flavescens | diphosphate + ? | - |
? | |
| dimethylallyl diphosphate + leachianone G | the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone characteristic to this plant | Sophora flavescens | diphosphate + sophoraflavanone G | - |
? | |
| dimethylallyl diphosphate + leachianone G | the enzyme is a prenyl side-chain elongation enzyme, the enzyme transfers a dimethylallyl group to the 2'' position of another dimethylallyl group attached at position 8 of leachianone G to form sophoraflavanone G, a branched monoterpenoid-conjugated flavanone, the enzyme is absolutely specific for dimethylallyl diphosphate as prenyl donor and highly specific for leachianone G, the 2'-hydroxy function in leachianone G is indispensable to the activity | Sophora flavescens | diphosphate + sophoraflavanone G | - |
? | |
| additional information | no activity with isopentenyl diphosphate and geranyl diphosphate as prenyl donors, and with kenusanone I, 8-dimethylallylnaringenin, and isovabachin as acceptor substrates, overview | Sophora flavescens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| leachianone G 2''-dimethylallyltransferase | - |
Sophora flavescens |
| LG 2''-dimethylallyltransferase | - |
Sophora flavescens |
| LGDT | - |
Sophora flavescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sophora flavescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Sophora flavescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 10 | 50% of maximal activity within this range, almost inactive at pH 6.8, pH profile, overview | Sophora flavescens |
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