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Literature summary for 2.5.1.70 extracted from

  • Kawasaki, T.; Koeduka, T.; Sugiyama, A.; Sasaki, K.; Linley, P.; Shitan, N.; Kumano, T.; Yamamoto, H.; Ezura, H.; Kuzuyama, T.; Yazaki, K.
    Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits (2014), Plant Biotechnol., 31, 567-571.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
gene N8DT1, recombinant expression in Solanum lycopersicum transgenic plants contitutively using CaMV 35S promoter Sophora flavescens
gene N8DT2, recombinant expression in Solanum lycopersicum transgenic plants contitutively using CaMV 35S promoter Sophora flavescens

Protein Variants

Protein Variants Comment Organism
additional information production of prenylated flavonoids in Solanum lycopersicum as a host plant by means of metabolic engineering through recombinant overexpression of both the naringenin 8-dimethylallyltransferase (N8DT) gene, encoding a prenyltransferase from Sophora flavescens, and the chalcone isomerase (CHI) gene from Nicotiana tabacum cv. Samsun-NN, in transgenic tomato lines. Production of 8-dimethylallyl naringenin in the double transformants, while the production level is lower than N8DT single transformants. Tomato fruits overexpressing both N8DT and CHI genes accumulate high levels of rutin compared with wild-type tomato Sophora flavescens

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the membrane-intrinsic enzyme protein contains a transit peptide sequence at the N-terminus that localizes it to the plastid even when expressed ectopically in heterologous host plants Sophora flavescens 9507
-
membrane
-
 Sophora flavescens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + (-)-(2S)-naringenin Sophora flavescens
-
 diphosphate + sophoraflavanone B
-
 ?

Organism

Organism UniProt Comment Textmining
Sophora flavescens B1B3P3 N8DT1; gene N8DT1
-
Sophora flavescens B1B5P4 N8DT2; gene N8DT2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + (-)-(2S)-naringenin
-
 Sophora flavescens diphosphate + sophoraflavanone B
-
 ?

Synonyms

Synonyms Comment Organism
N8DT1
-
 Sophora flavescens
N8DT2
-
 Sophora flavescens
naringenin 8-dimethylallyltransferase/chalcone isomerase
-
 Sophora flavescens

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthetic pathway of prenylflavonoid and rutin Sophora flavescens
additional information Tomato fruits overexpressing both N8DT and CHI genes accumulate high levels of rutin compared with wild-type tomato Sophora flavescens