Cloned (Comment) | Organism |
---|---|
for the expression of AbMurA, the plasmid is introduced into C41(DE3) cells | Acinetobacter baumannii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | 50 mM, 3fold inhibition | Acinetobacter baumannii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.062 | - |
UDP-N-acetyl-alpha-D-glucosamine | pH 7.5, 37°C | Acinetobacter baumannii | |
1.806 | - |
phosphoenolpyruvate | pH 7.5, 37°C | Acinetobacter baumannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Acinetobacter baumannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | Acinetobacter baumannii | the enzyme catalyzes the first committed step of peptidoglycan biosynthesis | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter baumannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | - |
Acinetobacter baumannii | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? | |
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine | the enzyme catalyzes the first committed step of peptidoglycan biosynthesis | Acinetobacter baumannii | phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Acinetobacter baumannii |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Acinetobacter baumannii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Acinetobacter baumannii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acinetobacter baumannii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
11.7 | - |
pH 7.5, 37°C | Acinetobacter baumannii | fosfomycin |
General Information | Comment | Organism |
---|---|---|
drug target | the lack of a counterparts in eukaryotes suggests the enzyme to be a promising drug target | Acinetobacter baumannii |
metabolism | the enzyme catalyzes the first committed step of peptidoglycan biosynthesis. MurA is indispensable for cell integrity | Acinetobacter baumannii |