Literature summary for 2.5.1.7 extracted from

Han, S.G.; Lee, W.K.; Jin, B.S.; Lee, K.I.; Lee, H.H.; Yu, Y.G.
Identification of novel irreversible inhibitors of UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Haemophilus influenzae (2013), J. Microbiol. Biotechnol., 23, 329-334.

Search for more literature for 2.5.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta2(DE3) cells	Haemophilus influenzae

Protein Variants

Protein Variants	Comment	Organism
C117D	the mutant is not inhibited by fosfomycin	Haemophilus influenzae

Inhibitors

Inhibitors	Comment	Organism	Structure
2-chloro-3-(4-methoxyphenoxy)naphthalene-1,4-dione	-	Haemophilus influenzae
6-chloro-7-(4-methoxyphenoxy)quinoline-5,8-dione	-	Haemophilus influenzae
7-chloro-6-(4-hydroxyphenoxy)quinoline-5,8-dione	-	Haemophilus influenzae
fosfomycin	-	Haemophilus influenzae
methyl 1,4-dihydroxynaphthalene-2-carboxylate	-	Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46300	-	-	Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine	Haemophilus influenzae	-	phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine	-	?

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA affinity column chromatography	Haemophilus influenzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine	-	Haemophilus influenzae	phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine	-	?

Subunits

Subunits	Comment	Organism
?	x * 46300, calculated from amino acid sequence	Haemophilus influenzae

Synonyms

Synonyms	Comment	Organism
MurA	-	Haemophilus influenzae
UDP-N-acetylglucosamine enolpyruvyl transferase	-	Haemophilus influenzae

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000053	-	pH and temperature not specified in the publication	Haemophilus influenzae	6-chloro-7-(4-methoxyphenoxy)quinoline-5,8-dione
0.000064	-	pH and temperature not specified in the publication	Haemophilus influenzae	7-chloro-6-(4-hydroxyphenoxy)quinoline-5,8-dione
0.00071	-	pH and temperature not specified in the publication	Haemophilus influenzae	2-chloro-3-(4-methoxyphenoxy)naphthalene-1,4-dione
0.0087	-	pH and temperature not specified in the publication	Haemophilus influenzae	methyl 1,4-dihydroxynaphthalene-2-carboxylate

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)