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Literature summary for 2.5.1.7 extracted from

  • Han, S.; Jin, B.; Lee, W.; Yu, Y.
    Kinetic properties of wild-type and C117D mutant UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Haemophilus influenzae (2011), Bull. Korean Chem. Soc., 32, 2549-2552.
No PubMed abstract available
Search for more literature for 2.5.1.7

Protein Variants

Protein Variants Comment Organism
C117D the mutation shifts ithe optimum pH from 7.8 to 6.0. The mutant is not inhibited by 1 mM fosfomycin Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
fosfomycin more than 90% inhibition at 0.2 mM Haemophilus influenzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 phosphoenolpyruvate wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae
0.031
-
 UDP-N-acetyl-D-glucosamine wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae
0.15
-
 phosphoenolpyruvate mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
0.16
-
 UDP-N-acetyl-D-glucosamine mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and gel filtration Haemophilus influenzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.91
-
 mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
4.7
-
 wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Haemophilus influenzae UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
MurA
-
 Haemophilus influenzae
UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 phosphoenolpyruvate mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
0.68
-
 UDP-N-acetyl-D-glucosamine mutant enzyme C117D, at pH 6.0 and 25°C Haemophilus influenzae
3.5
-
 phosphoenolpyruvate wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae
3.5
-
 UDP-N-acetyl-D-glucosamine wild type enzyme, at pH 7.8 and 25°C Haemophilus influenzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Haemophilus influenzae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 recombinant enzyme, enzyme activity gradually decreases when the pH is below 5.5 or above 10.0 Haemophilus influenzae