Cloned (Comment) | Organism |
---|---|
coding region of Haemophilus influenzae MurA is cloned into the NdeI and XhoI sites of pET21a to generate an expression vector for MurA, the expression vector is transformed into Escherichia coli Rosetta2 (DE3), and the recombinant MurA is expressed | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ebselen | inhibitor covalently modifies the cysteine residue near the active site-loop of MurA, modification changes the open conformation of MurA to a more closed configuration, when compound is incubated for 30 min with dithiothreitol, its inhibitory activity against Haemophilus influenzae MurA is completely eliminated | Haemophilus influenzae | |
fosfomycin | - |
Haemophilus influenzae | |
thimerosal | inhibitor covalently modifies the cysteine residue near the active site-loop of MurA, modification changes the open conformation of MurA to a more closed configuration, when compound is incubated for 30 min with dithiothreitol, its inhibitory activity against Haemophilus influenzae MurA is completely eliminated | Haemophilus influenzae | |
thiram | inhibitor covalently modifies the cysteine residue near the active site-loop of MurA, modification changes the open conformation of MurA to a more closed configuration, when compound is incubated for 30 min with dithiothreitol, its inhibitory activity against Haemophilus influenzae MurA is completely eliminated | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P45025 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
MurA | - |
Haemophilus influenzae |
UDP-N-acetylglucosamine enolpyruvyl transferase | - |
Haemophilus influenzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Haemophilus influenzae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0001 | - |
50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM | Haemophilus influenzae | ebselen | |
0.0004 | - |
50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM | Haemophilus influenzae | thimerosal | |
0.0007 | - |
50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM | Haemophilus influenzae | thiram | |
0.04 | - |
50 mM Tris-HCl, pH 7.8, compound displays an anti-MurA activity that is above 90% at a concentration below 0.002 mM | Haemophilus influenzae | fosfomycin |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme catalyzes the first step of bacterial cell wall synthesis | Haemophilus influenzae |