Protein Variants | Comment | Organism |
---|---|---|
C115S | - |
Enterobacter cloacae |
C251S | site-directed mutagenesis, Cys251 is not involved in the catalysis, unaltered biochemical properties | Enterobacter cloacae |
C354S | site-directed mutagenesis, Cys354 is not involved in the catalysis, unaltered biochemical properties | Enterobacter cloacae |
C381S | site-directed mutagenesis, Cys381 is not involved in the catalysis, unaltered biochemical properties | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosfomycin | binds covalently to Cys115 | Enterobacter cloacae | |
iodoacetamide | inhibition by alkylation of the active site Cys155, pH-dependent, no alkylation below pH 7.0, maximum alkylation at pH 9.0 | Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km values of mutant enzymes for the substrates | Enterobacter cloacae | |
0.008 | - |
phosphoenolpyruvate | wild-type enzyme | Enterobacter cloacae | |
0.08 | - |
UDP-GlcNAc | wild-type enzyme | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
MurA | - |
Enterobacter cloacae | - |
recombinant purified enzyme | - |
Enterobacter cloacae DSM 30054 | - |
MurA | - |
Purification (Comment) | Organism |
---|---|
covalent adduct of enzyme and phosphoenolpyruvate | Enterobacter cloacae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | mechanism | Enterobacter cloacae | |
phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | Cys115 in the active site can act as proton donor, necessary for activity, or as a nucleophile | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae DSM 30054 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
UDP-N-acetylglucosamine enolpyruvyltransferase | - |
Enterobacter cloacae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat values of mutant enzymes for the substrates | Enterobacter cloacae | |
3 | - |
phosphoenolpyruvate | wild-type enzyme | Enterobacter cloacae | |
3 | - |
UDP-GlcNAc | wild-type enzyme | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
determination of pKa value for Cys115: 8.3 | Enterobacter cloacae |