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Literature summary for 2.5.1.7 extracted from
- Lysine 22 in UDP-N-acetylglucosamine enolpyruvyl transferase from Enterobacter cloacae is crucial for enzymatic activity and the formation of covalent adducts with the substrate phosphoenolpyruvate and the antibiotic fosfomycin (1999), Biochemistry, 38, 13162-13169.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | included in the assay reaction mixture | Enterobacter cloacae |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a target for the antibiotic fosfomycin | Enterobacter cloacae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutants in Escherichia coli | Enterobacter cloacae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C115S | - |
Enterobacter cloacae |
| K22E | site-directed mutagenesis, exchange of conserved Lys residue located near the active site and involved in substrate binding leading to conformational changes, shows less than 0.5% activity compared to the wild-type, altered UDP-GlcNAc binding, highly reduced formation of covalent adduct between active site Cys115 and phosphoenolpyruvate or inhibitor fosfomycin | Enterobacter cloacae |
| K22R | site-directed mutagenesis, exchange of conserved Lys residue located near the active site and involved in substrate binding leading to conformational changes, shows less than 0.5% activity compared to the wild-type, slightly reduced formation of covalent adduct between active site Cys115 and phosphoenolpyruvate or inhibitor fosfomycin | Enterobacter cloacae |
| K22V | site-directed mutagenesis, exchange of conserved Lys residue located near the active site and involved in substrate binding leading to conformational changes, shows less than 0.5% activity compared to the wild-type, reduced formation of covalent adduct between active site Cys115 and phosphoenolpyruvate or inhibitor fosfomycin | Enterobacter cloacae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fosfomycin | - |
Enterobacter cloacae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | P33038 | MurA | - |
| Enterobacter cloacae DSM 30054 | P33038 | MurA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutants from Escherichia coli | Enterobacter cloacae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine | Lys22 is located near the active site and involved in substrate binding | Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of K22 mutants compared to wild-type in forward and reverse reaction, coupled assay | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | substrate binding structure | Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | thermodynamic parameters of substrate binding, wild-type, C115S and K22 mutants | Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | substrate binding structure | Enterobacter cloacae DSM 30054 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | thermodynamic parameters of substrate binding, wild-type, C115S and K22 mutants | Enterobacter cloacae DSM 30054 | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure | Enterobacter cloacae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Enterobacter cloacae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Enterobacter cloacae |
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