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Literature summary for 2.5.1.7 extracted from

  • Wanke, C.; Falchetto, R.; Amrhein, N.
    The UDP-N-acetylglucosamine 1-carboxyvinyl-transferase of Enterobacter cloacae. Molecular cloning, sequencing of the gene and overexpression of the enzyme (1992), FEBS Lett., 301, 271-276.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, functional overexpression in Escherichia coli strain JM105 Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
nondenaturing PAGE Enterobacter cloacae
42100
-
42100, SDS-PAGE Enterobacter cloacae
44780
-
MW deduced from amino acid sequence Enterobacter cloacae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Enterobacter cloacae enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Enterobacter cloacae DSM 30054 enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
Nr. 30054
-
Enterobacter cloacae DSM 30054
-
Nr. 30054
-

Purification (Commentary)

Purification (Comment) Organism
-
Enterobacter cloacae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
purified native enzyme Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan Enterobacter cloacae phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?
phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan Enterobacter cloacae DSM 30054 phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine
-
?

Subunits

Subunits Comment Organism
monomer 42100, SDS-PAGE Enterobacter cloacae

Synonyms

Synonyms Comment Organism
enol-pyruvyltransferase
-
Enterobacter cloacae