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Literature summary for 2.5.1.7 extracted from
- Pyruvate-uridine diphospho-N-acetylglucosamine transferase. Purification to homogeneity and feedback inhibition (1975), J. Biol. Chem., 250, 3185-3192.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP-N-acetylmuramyl-L-Ala-D-Glu-meso-alpha,epsilon-diaminopimelic acid | inhibitor binding site is distinct from active site | Enterobacter cloacae |  |
| uridine diphospho-N-acetylmuramyl-L-Ala-D-gamma-Glu-meso-alpha,epsilon-diaminopimelic-acid-D-Ala-D-Ala | inhibitor binding site is distinct from active site | Enterobacter cloacae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Enterobacter cloacae |
| 41000 | - |
1 * 41000, SDS-PAGE | Enterobacter cloacae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | Enterobacter cloacae | enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterobacter cloacae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.9 | - |
- |
Enterobacter cloacae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, loss of activity after 1 week | Enterobacter cloacae |
| 4°C, in 80% ammonium sulfate, loss of activity after 1 week | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | - |
Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | i.e. UDP-N-acetyl-D-glucosamine-enolpyruvate | ? | |
| phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine | enzyme catalyzes the first committed step in the biosynthesis of bacterial cell wall peptidoglycan | Enterobacter cloacae | phosphate + UDP-N-acetyl-3-(1-carboxyvinyl)-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41000, SDS-PAGE | Enterobacter cloacae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
uridine diphospho-N-acetylmuramyl-L-Ala-D-gamma-Glu-meso-alpha,epsilon-diaminopimelic-acid-D-Ala-D-Ala | - |
Enterobacter cloacae | |
| 4.9 | 6.6 | UDP-N-acetylmuramyl-L-Ala-D-Glu-meso-alpha,epsilon-diaminopimelic acid | - |
Enterobacter cloacae | |
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