Cloned (Comment) | Organism |
---|---|
phage lambda cDNA library screening, DNA and amino acid sequence determination and analysis, functional overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90 | Tanacetum cinerariifolium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-stae kinetics, the His6-tag of the recombinant enzyme does not influence the kinetics | Tanacetum cinerariifolium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Tanacetum cinerariifolium | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, 1-10 mM, can be substituted by Mn2+, best divalent cation | Tanacetum cinerariifolium | |
Mn2+ | activates, can substitute for Mg2+, optimal at 1 mM, less effective than Mg2+ | Tanacetum cinerariifolium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation | Tanacetum cinerariifolium |
41200 | - |
1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation | Tanacetum cinerariifolium |
45358 | - |
1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation | Tanacetum cinerariifolium |
66000 | - |
recombinant enzyme, gel filtration | Tanacetum cinerariifolium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 dimethylallyl diphosphate | Tanacetum cinerariifolium | - |
diphosphate + chrysanthemyl diphosphate | - |
? | |
additional information | Tanacetum cinerariifolium | irregular monoterpenes are common in Chrysanthemum cinerariaefolium and related members of the asteraceae family, CPP is an intermediate in the biosynthesis of the pyrethrin ester insecticides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tanacetum cinerariifolium | P0C565 | i.e. Tanacetum cinerariifolium, dalmatian daisy | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the pre-enzyme contains a putative plastidial targeting sequence | Tanacetum cinerariifolium |
Purification (Comment) | Organism |
---|---|
native enzyme from immature flowers by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion chromatography and hydroxylapatite chromatography to apparent homogeneity, recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by ammonium sulfate fractionation and nickel affinity chromatography | Tanacetum cinerariifolium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | immature | Tanacetum cinerariifolium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00267 | - |
purified recombinant enzyme | Tanacetum cinerariifolium |
Storage Stability | Organism |
---|---|
-20°C, purified native enzyme in 30% glycerol for about 72 h with 20% decrease in activity | Tanacetum cinerariifolium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 dimethylallyl diphosphate | - |
Tanacetum cinerariifolium | diphosphate + chrysanthemyl diphosphate | - |
? | |
2 dimethylallyl diphosphate | - |
Tanacetum cinerariifolium | diphosphate + chrysanthemyl diphosphate | product analysis, chrysanthemyl diphosphate is an irregular monoterpene with a non-head-to-tail or irregular c1'-2-3 linkage between isoprenoid units | ? | |
additional information | irregular monoterpenes are common in Chrysanthemum cinerariaefolium and related members of the asteraceae family, CPP is an intermediate in the biosynthesis of the pyrethrin ester insecticides | Tanacetum cinerariifolium | ? | - |
? | |
additional information | CPPase seems to be incapable of catalyzing 19-4 chain elongation despite its significant sequence homology to FPPase | Tanacetum cinerariifolium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45358, pre-protein, sequence calculation, 1 * 41000, recombinant enzyme, SDS-PAGE, 1 * 41200, recombinant enzyme, analytical sedimentation equilibrium ultracentrifugation | Tanacetum cinerariifolium |
Synonyms | Comment | Organism |
---|---|---|
CPPase | - |
Tanacetum cinerariifolium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tanacetum cinerariifolium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
dimethylallyl diphosphate | pH 7.5, 30°C, His-tagged or detagged recombinant enzyme | Tanacetum cinerariifolium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Tanacetum cinerariifolium |