Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Aeropyrum pernix |
overexpression in Escherichia coli Rosetta (DE3) | Aeropyrum pernix |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method | Aeropyrum pernix |
purified recombinant enzyme, optimization of crystallization method, hanging drop vapour diffusion method, enzyme in 50 mM potassium phosphate buffer pH 7.5, containing 0.2 mM pyridoxal 5'-phosphate and 0.05% sodium azide, 0.0015 ml of enzyme and reservoir solution, containing 0.1 M sodium cacodylate pH 7.4, 0.1 M sodium acetate and 30% v/v PEG 8000, are equilibrated against 0.5 ml of reservoir solution at 295°C, 2 weeks, cryoprotection by 10% v/v glycerol, X-ray diffraction structure determination and analysis at 2.2 A resolution | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aeropyrum pernix | L-cysteine biosynthesis | ? | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | Aeropyrum pernix | - |
L-cysteine + acetate | - |
? | |
O-phospho-L-serine + hydrogen sulfide | Aeropyrum pernix | - |
L-cysteine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Aeropyrum pernix |
recombinant OASS | Aeropyrum pernix |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
O-phospho-L-serine + hydrogen sulfide = L-cysteine + phosphate | a pyridoxal-phosphate protein, the enzyme from Aeropyrum pernix acts on both O-phospho-L-serine and O3-acetyl-L-serine, in contrast with EC 2.5.1.47, cysteine synthase, which acts only on O3-acetyl-L-serine | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | L-cysteine biosynthesis | Aeropyrum pernix | ? | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | - |
Aeropyrum pernix | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + sulfide | - |
Aeropyrum pernix | L-cysteine + acetic acid | - |
? | |
O-phospho-L-serine + hydrogen sulfide | - |
Aeropyrum pernix | L-cysteine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
APE1586 | Aeropyrum pernix K1, open reading frame | Aeropyrum pernix |
O-acetyl-L-serine sulfhydrylase | - |
Aeropyrum pernix |
O-acetylserine sulfhydrylase | - |
Aeropyrum pernix |
O-phosphoserine(thiol)-lyase | - |
Aeropyrum pernix |
OASS | - |
Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Aeropyrum pernix |