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Literature summary for 2.5.1.63 extracted from
- Identification and characterisation of a fluorinase from Actinopolyspora mzabensis (2020), Protein Expr. Purif., 166, 105508 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichichia coli | Actinopolyspora mzabensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, about 31% residual activity | Actinopolyspora mzabensis |  |
| EDTA | 1 mM, about 9% loss of activity | Actinopolyspora mzabensis | |
| additional information | not inhibitory: Mg2+, Mn2+, Co2+ and Fe2+ | Actinopolyspora mzabensis | |
| Zn2+ | 1 mM, about 31% residual activity | Actinopolyspora mzabensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM, 9.5% stimulation | Actinopolyspora mzabensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinopolyspora mzabensis | A0A1G9FQX8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recovery from inclusion bodies after heterologous expression in Escherichia coli | Actinopolyspora mzabensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.44 | - |
pH 7.2, 65°C | Actinopolyspora mzabensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, 2 months, 26% residual activity | Actinopolyspora mzabensis |
| 4°C, 2 months, 84% residual activity | Actinopolyspora mzabensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + fluoride | - |
Actinopolyspora mzabensis | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SAMN04487820_1174 | - |
Actinopolyspora mzabensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Actinopolyspora mzabensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
more than 80% of maximum activity | Actinopolyspora mzabensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Actinopolyspora mzabensis |
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Release 2023.1 (January 2023)
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