Literature summary for 2.5.1.63 extracted from

Tu, C.; Zhou, J.; Peng, L.; Man, S.; Ma, L.
Self-assembled nano-aggregates of fluorinases demonstrate enhanced enzymatic activity, thermostability and reusability (2020), Biomater. Sci., 8, 648-656 .

Search for more literature for 2.5.1.63

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces xinghaiensis

Protein Variants

Protein Variants	Comment	Organism
additional information	fusion of self-assembling amphipathic peptides into the C-terminus of fluorinase leads to proteins capable of self-assembly to form nano-sized particles with different dimensions in aqueous solutions. Peptide LLLLLLKD-tagged enzyme displays improved enzyme activity, thermostability and reusability	Streptomyces xinghaiensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0351	-	S-adenosyl-L-methionine	(LELELKLK)2-tagged enzyme, pH 6.0, 40°C	Streptomyces xinghaiensis
0.0362	-	S-adenosyl-L-methionine	wild-type, pH 6.0, 40°C	Streptomyces xinghaiensis
0.0411	-	S-adenosyl-L-methionine	LLLLLLKD-tagged enzyme, pH 6.0, 50°C	Streptomyces xinghaiensis
0.0721	-	S-adenosyl-L-methionine	EWLKAFYEKVLEKLKELF-tagged enzyme, pH 6.0, 60°C	Streptomyces xinghaiensis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces xinghaiensis	A0A068VNW5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + fluoride	-	Streptomyces xinghaiensis	5'-deoxy-5'-fluoroadenosine + L-methionine	-	?

Synonyms

Synonyms	Comment	Organism
FlA4	-	Streptomyces xinghaiensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	wild-type	Streptomyces xinghaiensis
50	-	peptide LLLLLLKD-tagged enzyme	Streptomyces xinghaiensis
60	-	peptide EWLKAFYEKVLEKLKELF-tagged enzyme	Streptomyces xinghaiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	half-life of wild-type, (LELELKLK)2-tagged enzyme is 52 and 55.9 min, respectively	Streptomyces xinghaiensis
50	-	half-life of wild-type, (LELELKLK)2-tagged enzyme, LLLLLLKD-tagged enzyme is 1.12, 3.28 and 82.5 min, respectively	Streptomyces xinghaiensis
60	-	half-life of LLLLLLKD-tagged enzyme and EWLKAFYEKVLEKLKELF-tagged enzyme is 0.72 and 2.49 min, respectively	Streptomyces xinghaiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0022	-	S-adenosyl-L-methionine	(LELELKLK)2-tagged enzyme, pH 6.0, 40°C	Streptomyces xinghaiensis
0.0026	-	S-adenosyl-L-methionine	wild-type, pH 6.0, 40°C	Streptomyces xinghaiensis
0.0034	-	S-adenosyl-L-methionine	LLLLLLKD-tagged enzyme, pH 6.0, 50°C	Streptomyces xinghaiensis
0.0047	-	S-adenosyl-L-methionine	EWLKAFYEKVLEKLKELF-tagged enzyme, pH 6.0, 60°C	Streptomyces xinghaiensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Streptomyces xinghaiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.062	-	S-adenosyl-L-methionine	(LELELKLK)2-tagged enzyme, pH 6.0, 40°C	Streptomyces xinghaiensis
0.065	-	S-adenosyl-L-methionine	EWLKAFYEKVLEKLKELF-tagged enzyme, pH 6.0, 60°C	Streptomyces xinghaiensis
0.076	-	S-adenosyl-L-methionine	wild-type, pH 6.0, 40°C	Streptomyces xinghaiensis
0.082	-	S-adenosyl-L-methionine	LLLLLLKD-tagged enzyme, pH 6.0, 50°C	Streptomyces xinghaiensis

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Release 2023.1 (January 2023)