Literature summary for 2.5.1.63 extracted from

Sun, H.; Yeo, W.L.; Lim, Y.H.; Chew, X.; Smith, D.J.; Xue, B.; Chan, K.P.; Robinson, R.C.; Robins, E.G.; Zhao, H.; Ang, E.L.
Directed evolution of a fluorinase for improved fluorination efficiency with a non-native substrate (2016), Angew. Chem. Int. Ed. Engl., 55, 14277-14280 .

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Protein Variants

Protein Variants	Comment	Organism
A279Y	about 3fold improved conversion of the non-native substrate 5'-deoxy-5'-chloroadenosine into 5'-fluoro-5'-deoxyadenosine. Mutant displays an improved kcat value in the conversion of 5'-deoxy-5'-chloroadenosine into S-adenosyl-L-methionine but a reduced kcat value in the conversion of S-adenosyl-L-methionine into 5'-fluoro-5'-deoxyadenosine	Streptomyces sp. MA37
F213Y/A279L	about 3fold improved conversion of the non-native substrate 5'-deoxy-5'-chloroadenosine into 5'-fluoro-5'-deoxyadenosine. Mutant displays an improved kcat value in the conversion of 5'-deoxy-5'-chloroadenosine into S-adenosyl-L-methionine but a reduced kcat value in the conversion of S-adenosyl-L-methionine into 5'-fluoro-5'-deoxyadenosine	Streptomyces sp. MA37

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0084	-	5'-deoxy-5'-chloroadenosine	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0092	-	S-adenosyl-L-methionine	mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0095	-	5'-deoxy-5'-chloroadenosine	mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0222	-	S-adenosyl-L-methionine	mutant A279Y, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0346	-	S-adenosyl-L-methionine	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0593	-	5'-deoxy-5'-chloroadenosine	mutant A279Y, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. MA37	W0W999	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-deoxy-5'-chloroadenosine + L-methionine	-	Streptomyces sp. MA37	S-adenosyl-L-methionine + chloride	-	r
5'-deoxy-5'-chloroadenosine + L-selenomethionine	use of L-selenomethionine increases the yield by around 2fold	Streptomyces sp. MA37	S-adenosyl-L-selenomethionine + chloride	-	r
additional information	by coupling the reactions, conversion of 5'-deoxy-5'-chloroadenosine to 5'-fluoro-5'-deoxyadenosine can be achieved	Streptomyces sp. MA37	?	-	-
S-adenosyl-L-methionine + fluoride	-	Streptomyces sp. MA37	5'-deoxy-5'-fluoroadenosine + L-methionine	-	r

Synonyms

Synonyms	Comment	Organism
FlA1	-	Streptomyces sp. MA37

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
42	-	-	Streptomyces sp. MA37

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0003	-	S-adenosyl-L-methionine	mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0018	-	S-adenosyl-L-methionine	mutant A279Y, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0022	-	5'-deoxy-5'-chloroadenosine	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0037	-	S-adenosyl-L-methionine	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0088	-	5'-deoxy-5'-chloroadenosine	mutant A279Y, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.0643	-	5'-deoxy-5'-chloroadenosine	mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.043	-	S-adenosyl-L-methionine	mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.08	-	S-adenosyl-L-methionine	mutant A279Y, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.108	-	S-adenosyl-L-methionine	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.149	-	5'-deoxy-5'-chloroadenosine	mutant A279Y, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
0.268	-	5'-deoxy-5'-chloroadenosine	wild-type, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37
6.798	-	5'-deoxy-5'-chloroadenosine	mutant F213Y/A279L, pH not specified in the publication, temperature not specified in the publication	Streptomyces sp. MA37

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Release 2023.1 (January 2023)