Crystallization (Comment) | Organism |
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purified recombinant enzyme, free from tags or bound adenosine, complexed to 2'-deoxy-5'-fluoroadenosine, 4 mg/ml protein is incubated with 20 mM ligand at 25°C for 4 h, followed by crystallization using vapour diffusion against a reservoir containing 30% PEG 1000, 0.1 M phosphate-citrate pH 4.5, 0.2 M Li2SO4, X-ray diffraction structure determination and analysis at 2.4 A resolution, molecular replacement | Streptomyces cattleya |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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S-adenosyl-L-methionine + fluoride | Streptomyces cattleya | first committed enzymatic step on the biosynthetic pathway to the fluorometabolites fluoroacetate and 4-fluorothreonine | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
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Streptomyces cattleya | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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2'-deoxyadenosyl-L-methionine + fluoride | C-F bond formation, substrate binding structure, NMR and mass spectrometry, overview | Streptomyces cattleya | 2'-deoxy-5'-fluoroadenosine + L-methionine | in the reverse reaction the enzyme shows 10% activity with the 2'-deoxy compound compared to the activity with the 5'-derivative | r | |
additional information | substrate specificity, the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation, overview, the enzyme catalyzes also transhalogenation from 2'-deoxy-5'-chloroadenosine to 2'-deoxy-fluoroadenosine via 2'-deoxy-SeAM, overview | Streptomyces cattleya | ? | - |
? | |
S-adenosyl-L-methionine + fluoride | first committed enzymatic step on the biosynthetic pathway to the fluorometabolites fluoroacetate and 4-fluorothreonine | Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
? | |
S-adenosyl-L-methionine + fluoride | C-F bond formation, substrate binding structure, NMR and mass spectrometry, overview | Streptomyces cattleya | 5'-deoxy-5'-fluoroadenosine + L-methionine | - |
r |
Synonyms | Comment | Organism |
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fluorinase | - |
Streptomyces cattleya |