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Literature summary for 2.5.1.62 extracted from

  • Shcherbakov, R.A.; Shalygo, N.V.
    Determination of chlorophyll synthetase activity in green tobacco leaves using exogenous Zn-chlorophyllide a (2006), J. Appl. Spectr., 73, 103-106.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chlorophyllide a + phytyl diphosphate Nicotiana tabacum the enzyme catalyzes the final step of chlorophyll a formation under in leaves of plants chlorophyll a + diphosphate
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Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
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-
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Source Tissue

Source Tissue Comment Organism Textmining
leaf green Nicotiana tabacum
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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spectrophotometric assay method development with Zn-chlorophyllide a as substrate, a method based on the spectral differences between Zn-chlorophyll a and chlorophyll a, overview Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorophyllide a + phytyl diphosphate the enzyme catalyzes the final step of chlorophyll a formation under in leaves of plants Nicotiana tabacum chlorophyll a + diphosphate
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?
chlorophyllide a + phytyl diphosphate chlorophyllide a possesses a central Mg2+ ion Nicotiana tabacum chlorophyll a + diphosphate
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Zn-chlorophyllide a + phytyl diphosphate Zn-chlorophyllide a possesses a central Zn2+ ion instead of Mg2+, can be used as as the substrate for chlorophyll synthetase in briefly illuminated etiolated rye leaves Nicotiana tabacum Zn-chlorophyll a + diphosphate
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?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
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assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
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assay at Nicotiana tabacum