Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.62 extracted from

  • Schmid, H.C.; Oster, U.; Kogel, J.; Lenz, S.; Rüdiger, W.
    Cloning and characterisation of chlorophyll synthase from Avena sativa (2001), Biol. Chem., 382, 903-911.
    View publication on PubMed
Search for more literature for 2.5.1.62

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Avena sativa

Protein Variants

Protein Variants Comment Organism
C109A mutant enzyme exhibits nearly no enzymatic activity, N-phenylmaleimide results in an additional decrease of activity Avena sativa
C130A mutant enzyme shows reduced activity and sensitivity to N-phenylmaleimide Avena sativa
C137A mutant enzyme is not impaired in enzymatic activity and shows the same inhibition by N-phenylmaleimide as the wild-type enzyme Avena sativa
C262A mutant enzyme is not impaired in enzymatic activity and shows the same inhibition by N-phenylmaleimide as the wild-type enzyme Avena sativa
C304A as active as the wild-type enzyme, mutant enzyme is not inhibited by N-phenylmaleimide Avena sativa
additional information deletion of the presequence yields a protein with full activity, even further deletion of the N-terminus including amino acid residues 1-87 results in a core protein that is still enzymatically active. Deletion of the 88 N-terminal residues yields a protein without enzymatic activity. At the C-terminus, only one residue H378 can be deleted without loss of activity, while deletion of S77 together with H378, and all shorter sequences show no activity Avena sativa
R151A mutant enzyme with 35% of the activity compared to wild-type enzyme Avena sativa
R161A mutant enzyme without activity Avena sativa
R284A mutant enzyme shows wild-type activity Avena sativa
R91A mutant enzyme without activity Avena sativa

Inhibitors

Inhibitors Comment Organism Structure
diacetyl
-
 Avena sativa
N-Phenylmaleimide since the wild-type enzyme and all other Cys-mutants with the exception of the mutant C304A are inhibited, it is concluded that the inhibitor binds to a non-essential Cys residue to abolish activity Avena sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, reconstitutes activity after treatment with EDTA Avena sativa
Mn2+ partially reconstitutes activity after treatment with EDTA Avena sativa
Zn2+ partially reconstitutes activity after treatment with EDTA Avena sativa

Organism

Organism UniProt Comment Textmining
Avena sativa
-
-
-