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Literature summary for 2.5.1.61 extracted from
- Purification, characterization, crystallisation and X-ray analysis of selenomethionine-labelled hydroxymethylbilane synthase from Escherichia coli (1993), Eur. J. Biochem., 211, 615-624.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| SeMet-labelled enzyme | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| SeMet-labelled enzyme | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | all, six, methionine residues are replaced by SeMet | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34268 | - |
1 * 34268, calculation from sequence of amino acid | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| porphobilinogen | Escherichia coli | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12, mutant PO1562 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| SeMet-labelled enzyme, i.e. [SeMet] HMBS, and wild-type enzyme | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Escherichia coli | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Escherichia coli | hydroxylmethylbilane + 4 NH3 | - |
? | |
| porphobilinogen | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34268, calculation from sequence of amino acid | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HMBS | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
SeMet-labelled enzyme | Escherichia coli |
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