Any feedback?
Literature summary for 2.5.1.61 extracted from
- Isolation, characterization and partial amino acid sequence of a chloroplast-localized porphobilinogen deaminase from pea (Pisum sativum L.) (1991), Biochim. Biophys. Acta, 1076, 29-36.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | CaCl2, weak | Pisum sativum |  |
| Co2+ | CoCl2, strong | Pisum sativum | |
| Fe2+ | FeSO4, strong | Pisum sativum | |
| Mg2+ | MgCl2, weak | Pisum sativum | |
| Mn2+ | MnCl2; strong inhibition at submillimolar concentrations | Pisum sativum | |
| Na+ | NaCl greater than 300 mM | Pisum sativum | |
| NH4+ | - |
Pisum sativum | |
| Zn2+ | ZnCl2, strong | Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Pisum sativum | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 45000, SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| porphobilinogen | Pisum sativum | - |
? | - |
? | |
| porphobilinogen | Glycine max | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
soya-bean | - |
| Pisum sativum | - |
pea | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| dye-ligand affinity chromatography | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Glycine max | - |
| leaf | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0813 | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or 4°C, unstable, loss of activity after several days | Pisum sativum |
| -20°C, crude plastide lysate is stable for at least 2 weeks | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Pisum sativum | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Glycine max | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Pisum sativum | hydroxylmethylbilane + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Glycine max | hydroxylmethylbilane + 4 NH3 | - |
? | |
| porphobilinogen | - |
Pisum sativum | ? | - |
? | |
| porphobilinogen | - |
Glycine max | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Pisum sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| porphobilinogen deaminase | - |
Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.2 | - |
Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | pH 6.5: 25% of maximal activity, pH 9.0: 50% of maximal activity | Pisum sativum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
