Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant Rab7 substrates with truncated C-terminal show that a lack of more than 3 residues leads to dramatic reduction in Rab prenylation efficiency of the enzyme, residual prenylation activity with 4-5 lacking residues, further reduction abolishes the ability of Rab7 to be an enzyme substrate | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E,10Z)-3,7,11-trimethyl-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodeca-2,6,10-trien-1-yl diphosphate + Rab7 GTPase | in vitro prenylation assay with 4 microM Rab7 wild type or mutants, 6 mircoM Rab escort protein, 6 microM enzyme, 50 mM HEPES buffer, pH 7.2, containing 50 mM NaCl, 5 mM dithioerythritol, 2 mM MgCl2, and 10 mircoM GDP, 25°C, 50 microM of the geranylgeranyl diphosphate analogue (3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,10-triem-1) diphosphate, end-point assay with 6 microM Rab7 wild type or mutants, 10 microM Rab escort protein, 6 microM enzyme in the same buffer mixture, and 40 microM (3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino)-dodeca-2,6,10-triem-1) diphosphate, 1.5 hours at 25°C | Homo sapiens | (2E,6E,10Z)-3,7,11-trimethyl-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodeca-2,6,10-trien-1-yl-Rab7 GTPase + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 2 * ?, computational model based on crystals of subcomplexes | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Rab geranylgeranyltransferase | - |
Homo sapiens |
RabGGTase | - |
Homo sapiens |