Activating Compound | Comment | Organism | Structure |
---|---|---|---|
reduced thioredoxin | increases the activity | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression in the Escherichia coli strain M15 | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
C113S | compared with the wild type enzyme the mutant enzyme is only weakly activated by PfTrx1. The mutation significantly decreases the affinity to the substrate L-methionine. The mutant enzyme shows higher affinity towards ATP when compared with the wild type | Plasmodium falciparum |
C187S | with regard to specific activity the mutant enzyme does not show major differences compared with the wild type enzyme. The mutant enzyme shows higher affinity towards ATP when compared with the wild type | Plasmodium falciparum |
C52S | compared with the wild type enzyme the mutant enzyme is only weakly activated by PfTrx1. With regard to specific activity the mutant enzyme does not show major differences compared with the wild type enzyme. The mutation significantly decreases the affinity to the substrate L-methionine and ATP | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NO | the enzyme is inhibited upon S-nitrosylation. S-Nitrosylation of the enzyme is mediated via several cysteine residues, including Cys52, Cys113 and Cys187. Nitrosylation is a reversible posttranslational modification upon nitrosative stress | Plasmodium falciparum | |
S-nitrosoglutathione | the enzyme is inhibited upon S-nitrosylation. S-Nitrosylation of the enzyme is not only mediated via a single cysteine but via several cysteine residues, including Cys52, Cys113 and Cys187 | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
L-methionine | pH 8.4, 37°C | Plasmodium falciparum | |
0.043 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
0.046 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
0.051 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
0.176 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
0.206 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
0.56 | - |
ATP | pH 8.4, 37°C | Plasmodium falciparum | |
0.68 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Plasmodium falciparum | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q7K6A4 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glutathionylation | the enzyme is inhibited upon glutathionylation | Plasmodium falciparum |
nitrosylation | the enzyme is inhibited upon S-nitrosylation. S-Nitrosylation of PfalMAT is mediated via several cysteine residues, including Cys52, Cys113 and Cys187. Nitrosylation is a reversible posttranslational modification upon nitrosative stress | Plasmodium falciparum |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Plasmodium falciparum | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
1.02 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
1.05 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
1.05 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
1.06 | - |
ATP | pH 8.4, 37°C | Plasmodium falciparum | |
1.06 | - |
L-methionine | pH 8.4, 37°C | Plasmodium falciparum | |
1.48 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
1.48 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of S-adenosylmethionine has downstream effects on polyamine metabolism and methylation reactions, and is an effective way to combat pathogenic microorganisms such as malaria parasites | Plasmodium falciparum |
metabolism | as a methyl group donor for biochemical reactions, the product S-adenosylmethionine plays a central metabolic role in most organisms | Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
1.9 | - |
ATP | pH 8.4, 37°C | Plasmodium falciparum | |
5.9 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
7.2 | - |
ATP | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
22.3 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
24.5 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum | |
31.2 | - |
L-methionine | pH 8.4, 37°C | Plasmodium falciparum | |
32.2 | - |
L-methionine | pH 8.4, 37°C, mutant enzyme | Plasmodium falciparum |