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Literature summary for 2.5.1.6 extracted from

  • Pretzel, J.; Gehr, M.; Eisenkolb, M.; Wang, L.; Fritz-Wolf, K.; Rahlfs, S.; Becker, K.; Jortzik, E.
    Characterization and redox regulation of Plasmodium falciparum methionine adenosyltransferase (2016), J. Biochem., 160, 355-367 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
reduced thioredoxin increases the activity Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expression in the Escherichia coli strain M15 Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C113S compared with the wild type enzyme the mutant enzyme is only weakly activated by PfTrx1. The mutation significantly decreases the affinity to the substrate L-methionine. The mutant enzyme shows higher affinity towards ATP when compared with the wild type Plasmodium falciparum
C187S with regard to specific activity the mutant enzyme does not show major differences compared with the wild type enzyme. The mutant enzyme shows higher affinity towards ATP when compared with the wild type Plasmodium falciparum
C52S compared with the wild type enzyme the mutant enzyme is only weakly activated by PfTrx1. With regard to specific activity the mutant enzyme does not show major differences compared with the wild type enzyme. The mutation significantly decreases the affinity to the substrate L-methionine and ATP Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
NO the enzyme is inhibited upon S-nitrosylation. S-Nitrosylation of the enzyme is mediated via several cysteine residues, including Cys52, Cys113 and Cys187. Nitrosylation is a reversible posttranslational modification upon nitrosative stress Plasmodium falciparum
S-nitrosoglutathione the enzyme is inhibited upon S-nitrosylation. S-Nitrosylation of the enzyme is not only mediated via a single cysteine but via several cysteine residues, including Cys52, Cys113 and Cys187 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
L-methionine pH 8.4, 37°C Plasmodium falciparum
0.043
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
0.046
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
0.051
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
0.176
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
0.206
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
0.56
-
ATP pH 8.4, 37°C Plasmodium falciparum
0.68
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Plasmodium falciparum
-
phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q7K6A4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glutathionylation the enzyme is inhibited upon glutathionylation Plasmodium falciparum
nitrosylation the enzyme is inhibited upon S-nitrosylation. S-Nitrosylation of PfalMAT is mediated via several cysteine residues, including Cys52, Cys113 and Cys187. Nitrosylation is a reversible posttranslational modification upon nitrosative stress Plasmodium falciparum

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Plasmodium falciparum phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.02
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
1.02
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
1.05
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
1.05
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
1.06
-
ATP pH 8.4, 37°C Plasmodium falciparum
1.06
-
L-methionine pH 8.4, 37°C Plasmodium falciparum
1.48
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
1.48
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum

General Information

General Information Comment Organism
malfunction depletion of S-adenosylmethionine has downstream effects on polyamine metabolism and methylation reactions, and is an effective way to combat pathogenic microorganisms such as malaria parasites Plasmodium falciparum
metabolism as a methyl group donor for biochemical reactions, the product S-adenosylmethionine plays a central metabolic role in most organisms Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
1.9
-
ATP pH 8.4, 37°C Plasmodium falciparum
5.9
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
7.2
-
ATP pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
22.3
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
24.5
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum
31.2
-
L-methionine pH 8.4, 37°C Plasmodium falciparum
32.2
-
L-methionine pH 8.4, 37°C, mutant enzyme Plasmodium falciparum