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Literature summary for 2.5.1.6 extracted from
- Isolation, expression, and characterization of S-adenosyl-L-methionine synthetase from Lactobacillus paraplantarum 72815 (2015), Food Sci. Biotechnol., 24, 1447-1451.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Lactiplantibacillus paraplantarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.47 | - |
L-methionine | at pH 8.5 and 40°C | Lactiplantibacillus paraplantarum |  |
| 2.84 | - |
ATP | at pH 8.5 and 40°C | Lactiplantibacillus paraplantarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Lactiplantibacillus paraplantarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | Lactiplantibacillus paraplantarum | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | Lactiplantibacillus paraplantarum 72815 | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus paraplantarum | - |
- |
- |
| Lactiplantibacillus paraplantarum 72815 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and gel filtration | Lactiplantibacillus paraplantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | - |
Lactiplantibacillus paraplantarum | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | - |
Lactiplantibacillus paraplantarum 72815 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Lactiplantibacillus paraplantarum |
| ? | x * 42663, calculated from amino acid sequence | Lactiplantibacillus paraplantarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S-adenosyl-L-methionine synthetase | - |
Lactiplantibacillus paraplantarum |
| SAM synthetase | - |
Lactiplantibacillus paraplantarum |
| SAM-s | - |
Lactiplantibacillus paraplantarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Lactiplantibacillus paraplantarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | more than 50% activity between 20 and 50°C. The activity is low below 20°C and above 55°C with less than 20% relative activity | Lactiplantibacillus paraplantarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
the enzyme is stable with a residual activity over 85% after incubation at 35°C for 2 h | Lactiplantibacillus paraplantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Lactiplantibacillus paraplantarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | more than 60% activity between pH 8.0 and 9.0. There is no activity below pH 6.0 | Lactiplantibacillus paraplantarum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Lactiplantibacillus paraplantarum | calculated from amino acid sequence | - |
4.7 |
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