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Literature summary for 2.5.1.6 extracted from
- Overexpression of methionine adenosyltransferase in Corynebacterium glutamicum for production of S-adenosyl-L-methionine (2016), Biotechnol. Appl. Biochem., 63, 679-689.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Corynebacterium glutamicum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
L-methionine | at pH 8.5 and 35°C | Corynebacterium glutamicum |  |
| 2.07 | - |
ATP | at pH 8.5 and 35°C | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Corynebacterium glutamicum |
| 44100 | - |
- |
Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | Corynebacterium glutamicum | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | Corynebacterium glutamicum ATCC 13032 | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | Q9K5E4 | - |
- |
| Corynebacterium glutamicum ATCC 13032 | Q9K5E4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, and gel filtration | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | - |
Corynebacterium glutamicum | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | - |
Corynebacterium glutamicum ATCC 13032 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Corynebacterium glutamicum |
| ? | x * 44100, calculated from amino acid sequence | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methionine adenosyltransferase | - |
Corynebacterium glutamicum |
| MetK | - |
Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Corynebacterium glutamicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | the activity of MetK does not change much when temperature is above 45°C, and 70% of the maximal activity is maintained at 50°C. At 60°C, the enzyme shows no methionine adenosyltransferase activity | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Corynebacterium glutamicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | the activity is significantly reduced at pH values below 6.0 or above 11.0 | Corynebacterium glutamicum |
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Release 2023.1 (January 2023)
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