Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method | Homo sapiens |
sitting drop vapor diffusion method, using | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A55D | the mutation reduces the enzyme activity by more than 50% | Homo sapiens |
D258G | the mutation reduces the enzyme activity by more than 50% | Homo sapiens |
I322M | the mutation reduces the enzyme activity by more than 50% | Homo sapiens |
R264H | the mutation almost completely abolishes enzyme activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Homo sapiens | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P31153 | isoform MAT2A | - |
Homo sapiens | Q00266 | isoform MAT1A | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, HiTrap HP-Q column chromatography, and Superdex 200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | isoform MAT1A | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Homo sapiens | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MAT1A | isoform | Homo sapiens |
MAT2A | isoform | Homo sapiens |
methionine adenosyltransferase | - |
Homo sapiens |
S-adenosylmethionine synthetase | - |
Homo sapiens |