Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris G115S cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 5% residual activity at 10 mM | Escherichia coli | |
Co2+ | about 45% residual activity at 10 mM | Escherichia coli | |
Cu2+ | complete inhibition at 10 mM | Escherichia coli | |
Fe2+ | about 15% residual activity at 10 mM | Escherichia coli | |
Mn2+ | about 80% residual activity at 10 mM | Escherichia coli | |
Ni2+ | about 1% residual activity at 10 mM | Escherichia coli | |
Zn2+ | about 10% residual activity at 10 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
L-methionine | at pH 8.5 and 45°C | Escherichia coli | |
1.72 | - |
ATP | at pH 8.5 and 45°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required | Escherichia coli | |
Mg2+ | required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Escherichia coli | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and phenyl Sepharose 6 column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.068 | - |
crude enzyme, at pH 8.5 and 45°C | Escherichia coli |
1.828 | - |
after 26.9fold purification, at pH 8.5 and 45°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Escherichia coli | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
MAT | - |
Escherichia coli |
methionine adenosyltransferase | - |
Escherichia coli |
pDS16 | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | about 20% activity at 20°C, about 30% activity at 30°C, about 80% activity at 35°C, about 90% activity at 40°C, 100% at 45°C, about 850% activity at 50°C, about 45% activity at 60°C, and no activity at 70°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | the enzyme activity remains stable after 2 h preincubation at 20-40°C in Tris-HCl (pH 8.9) | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | more than 50% activity between pH 7.5 and 10.0 | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
5.5 |