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Literature summary for 2.5.1.6 extracted from

  • Yao, G.; Qin, X.; Chu, J.; Wu, X.; Qian, J.
    Expression, purification, and characterization of a recombinant methionine adenosyltransferase pDS16 in Pichia pastoris (2014), Appl. Biochem. Biotechnol., 172, 1241-1253.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris G115S cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 5% residual activity at 10 mM Escherichia coli
Co2+ about 45% residual activity at 10 mM Escherichia coli
Cu2+ complete inhibition at 10 mM Escherichia coli
Fe2+ about 15% residual activity at 10 mM Escherichia coli
Mn2+ about 80% residual activity at 10 mM Escherichia coli
Ni2+ about 1% residual activity at 10 mM Escherichia coli
Zn2+ about 10% residual activity at 10 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
L-methionine at pH 8.5 and 45°C Escherichia coli
1.72
-
ATP at pH 8.5 and 45°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Escherichia coli
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Escherichia coli
-
phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and phenyl Sepharose 6 column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.068
-
crude enzyme, at pH 8.5 and 45°C Escherichia coli
1.828
-
after 26.9fold purification, at pH 8.5 and 45°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Escherichia coli phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
MAT
-
Escherichia coli
methionine adenosyltransferase
-
Escherichia coli
pDS16
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 about 20% activity at 20°C, about 30% activity at 30°C, about 80% activity at 35°C, about 90% activity at 40°C, 100% at 45°C, about 850% activity at 50°C, about 45% activity at 60°C, and no activity at 70°C Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 the enzyme activity remains stable after 2 h preincubation at 20-40°C in Tris-HCl (pH 8.9) Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 more than 50% activity between pH 7.5 and 10.0 Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
5.5